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Nodularin, microcystin, and the configuration of AddaJournal of the American Chemical Society, 110
- Publisher
- Oxford University Press
- Copyright
- © 1990 Federation of European Microbiological Societies
- ISSN
- 0378-1097
- eISSN
- 1574-6968
- DOI
- 10.1111/j.1574-6968.1990.tb04112.x
- Publisher site
- See Article on Publisher Site
Abstract
Summary An isoation procedure was developed to provide within one day microcystin-LR, a cyclic heptapeptide toxin from Microcystis aeruginosa PCC 7806. After ODS (octadecylsilyl) solid phase extraction, the crude toxin fraction was chromatographed using a strong anion exchange column. The toxin was eluted with 0.02 M ammonium bicarbonate. An at least 95% purity was revealed on HPLC separation by monitoring at 214 nm. Application of the procedure to the cyclic pentapeptide toxin nodulation from Nodularia spumigena AV2 was examined. Microcystis aeruginosa, Microcystin-LR, Nodularia spumigena, Nodularin, Rapid isolation, Anion exchange chromatography References [1] Botes D.B. Tuinman A.A. Wessels P.L. Viljoen C.C. Kruger H. ( 1984 ) J. Chem. Soc. Perkin Trans. , 1 , 2311 – 2318 . Crossref Search ADS [2] Falconer I.R. Beresfold A.M. Runnegar M.T.C. ( 1983 ) Med. J. Aust. , 1 , 511 – 514 . PubMed [3] Eriksson J.E. Meriluoto J.A.O. Kujari H.F. Österlund K. Fagerlund K. Hällborn L. ( 1988 ) Toxicon , 26 , 161 – 166 . Crossref Search ADS PubMed [4] Rinehart K.L. Harada K.I. Namikoshi M. Chen C. Harvis C.A. Munro M.H.G. Blunt J.W. Mulligan P.E. Beasley V.R. Dahlem A.M. Carmichael W.W. ( 1988 ) J. Am. Chem. Soc. , 110 , 8557 – 8558 . Crossref Search ADS [5] Sivonen K. Kononen K. Carmichael W.W. Dahlem A.M. Rinehart K.L. Kiviranta J. Niemelä S.I. ( 1989 ) Appl. Env. Microbiol. , 55 , 1990 – 1995 . [6] Krishnamurthy T. Carmichael W.W. Sarver E.W. ( 1986 ) Toxicon , 26 , 865 – 873 . Crossref Search ADS [7] Brooks W.P. Codd G.A. ( 1986 ) Lett. Appl. Microbiol. , 2 , 1 – 3 . Crossref Search ADS [8] Martin C. Codd G.A. Siegelman H.W. Weckesser J. ( 1989 ) Arch. Microbiol. , 152 , 90 – 94 . Crossref Search ADS [9] Dierstein R. Kaiser I. Weckesser J. ( 1988 ) FEMS Microbiol. Lett. , 49 , 143 – 147 . Crossref Search ADS [10] Dierstein R. Kaiser I. Weckesser J. Matern U. König W.A. Krebber R. Two closely related peptide toxins in the axenically grown cyanobacterium Microcystis aeruginosa PCC 7806 . Syst. Appl. Microbiol. , in press. [11] Harada K.-I. Suzuki M. Dahlem A.M. Beasley V.R. Carmichael W.W. Rinehart K.L. ( 1988 ) Toxicon , 26 , 433 – 439 . Crossref Search ADS PubMed This content is only available as a PDF. © 1990 Federation of European Microbiological Societies
Journal
FEMS Microbiology Letters – Oxford University Press
Published: Mar 1, 1990