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A simple and efficient method for isolating RNA from pine trees

A simple and efficient method for isolating RNA from pine trees Plant Molecular Biology Reporter pages 113-116 Volume 11(2) 1993 Commentary A Simple and Efficient Method for Isolating RNA from Pine Trees Key Words: phenolic compounds, pine, Pinus, polysaccharides, RNA extraction, RNase solating high quality RNA from pine trees is made difficult by the high concentrations of polysaccharides, phenolics and RNases asso- ciated with pine tissue. In seedlings subjected to stressful growth conditions the difficulty is exacerbated. To address this problem we have developed a rapid and facile modification of established techniques which allows preparation of total RNA from tissue within a few hours without the use of toxic and expensive chemicals such as phenol, guanidium isothiocyanate and guanidium hydrochloride or the need for ultracentrifugation. This method can be used on any tissue and is particularly effective on recalcitrant materials such as pine needles. Materials and Methods Solutions required. (Use diethylpyrocarbonate-treated water (Sambrook et al., 1989) for all solutions. Extraction buffer: 2% CTAB (hexadecyltrimethylammonium bromide) 2% PVP (polyvinylpyrrolidinone K 30) 100 mM Tris-HCl (pH 8.0) 25 mM EDTA 2.0 M NaC1 0.5g/L spermidine (mix and autoclave) 2% [3-mercaptoethanol (add just before use) Chloroform:isoamyl alcohol (24:1) Abbreviations: CTAB, hexadecyltrimethylammonium bromide; IAA, isoamyl alcohol; SSTE, defined in Materials and Methods. 114 Chang, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Reporter Springer Journals

A simple and efficient method for isolating RNA from pine trees

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References (5)

Publisher
Springer Journals
Copyright
Copyright © International Society for Plant Molecular Biology 1993
ISSN
0735-9640
eISSN
1572-9818
DOI
10.1007/bf02670468
Publisher site
See Article on Publisher Site

Abstract

Plant Molecular Biology Reporter pages 113-116 Volume 11(2) 1993 Commentary A Simple and Efficient Method for Isolating RNA from Pine Trees Key Words: phenolic compounds, pine, Pinus, polysaccharides, RNA extraction, RNase solating high quality RNA from pine trees is made difficult by the high concentrations of polysaccharides, phenolics and RNases asso- ciated with pine tissue. In seedlings subjected to stressful growth conditions the difficulty is exacerbated. To address this problem we have developed a rapid and facile modification of established techniques which allows preparation of total RNA from tissue within a few hours without the use of toxic and expensive chemicals such as phenol, guanidium isothiocyanate and guanidium hydrochloride or the need for ultracentrifugation. This method can be used on any tissue and is particularly effective on recalcitrant materials such as pine needles. Materials and Methods Solutions required. (Use diethylpyrocarbonate-treated water (Sambrook et al., 1989) for all solutions. Extraction buffer: 2% CTAB (hexadecyltrimethylammonium bromide) 2% PVP (polyvinylpyrrolidinone K 30) 100 mM Tris-HCl (pH 8.0) 25 mM EDTA 2.0 M NaC1 0.5g/L spermidine (mix and autoclave) 2% [3-mercaptoethanol (add just before use) Chloroform:isoamyl alcohol (24:1) Abbreviations: CTAB, hexadecyltrimethylammonium bromide; IAA, isoamyl alcohol; SSTE, defined in Materials and Methods. 114 Chang,

Journal

Plant Molecular Biology Reporter – Springer Journals

Published: Jun 1, 1993

Keywords: phenolic compounds; pine; Pinus; polysaccharides; RNA extraction; RNase

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