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- Publisher
- Oxford University Press
- Copyright
- Copyright © 2021 American Society of Plant Biologists
- ISSN
- 0032-0889
- eISSN
- 1532-2548
- DOI
- 10.1104/pp.44.10.1428
- Publisher site
- See Article on Publisher Site
Abstract
Abstract Ultracentrifugation of barley aleurone cells results in the stratification of organelles thus allowing for a quantitation of those organelles. Gibberellic acid (GA3)-stimulated α-amylase production in stratified cells is reduced by centrifugation at gravitational forces greater than 40,000g. Forces below 30,000g do not affect GA3-stimulated α-amylase production although stratification of organelles occurs at these forces. The ability of centrifuged cells to respond maximally to GA3 by producing α-amylase is related to the degree of redistribution of organelles within these cells. Thus, recovery of cells from centrifugation at forces below 30,000g is rapid, while recovery from forces above 40,000g is slow. 1 Supported by National Science Foundation grant GB-8332. This content is only available as a PDF. © 1969 American Society of Plant Biologists This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
Journal
Plant Physiology – Oxford University Press
Published: Oct 1, 1969