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Study of the Catalytic Effect of Copper(II)–Protein Complexes on Luminol-H2O2 Chemiluminescence Reaction and its Analytical Application

Study of the Catalytic Effect of Copper(II)–Protein Complexes on Luminol-H2O2 Chemiluminescence... Through flow injection analysis experiments, it was discovered that unsaturated complexes of Cu(II) with proteins had a much stronger catalytic effect on the luminol–H2O2 chemiluminescence reaction than did Cu(II). Based on this, a new rapid and sensitive method for determination of some proteins in the range 0.1–20.0 μg ml−1 was established. The detection limits were at the 0.03–0.05μg ml−1 level for bovine serum albumin (BSA), human serum albumín (HSA), human γ–globulin (γ–G), and egg albumin (EA). The method was used for the determination of proteins in human serum with satisfactory results. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Letters Taylor & Francis

Study of the Catalytic Effect of Copper(II)–Protein Complexes on Luminol-H2O2 Chemiluminescence Reaction and its Analytical Application

Li, Zhengping; Li, Ke'an; Tong, Shenyang
Analytical Letters , Volume 32 (5): 13 – Jan 1, 1999
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References (16)

Publisher
Taylor & Francis
Copyright
Copyright Taylor & Francis Group, LLC
ISSN
1532-236X
eISSN
0003-2719
DOI
10.1080/00032719908542864
Publisher site
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Abstract

Through flow injection analysis experiments, it was discovered that unsaturated complexes of Cu(II) with proteins had a much stronger catalytic effect on the luminol–H2O2 chemiluminescence reaction than did Cu(II). Based on this, a new rapid and sensitive method for determination of some proteins in the range 0.1–20.0 μg ml−1 was established. The detection limits were at the 0.03–0.05μg ml−1 level for bovine serum albumin (BSA), human serum albumín (HSA), human γ–globulin (γ–G), and egg albumin (EA). The method was used for the determination of proteins in human serum with satisfactory results.

Journal

Analytical Letters – Taylor & Francis

Published: Jan 1, 1999

Keywords: Protein; copper(II); complex; chemiluminescence; luminol; peroxide

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