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References (19)
-
L. Smith, J. Sanders, R. Kaiser, Peter Hughes, Chris Dodd, C. Connell, C. Heiner, S. Kent, L. Hood (1986)
Fluorescence detection in automated DNA sequence analysisNature, 321
-
R. Gibbs, P. Nguyen, L. Mcbride, S. Koepf, C. Caskey (1989)
Identification of mutations leading to the Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA.Proceedings of the National Academy of Sciences of the United States of America, 86 6
-
S. Scharf, G. Horn, H. Erlich (1986)
Direct cloning and sequence analysis of enzymatically amplified genomic sequences.Science, 233 4768
-
C. Caskey (1987)
Disease diagnosis by recombinant DNA methods.Science, 236 4806
-
M. Innis, K. Myambo, D. Gelfand, M. Brow (1988)
DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.Proceedings of the National Academy of Sciences of the United States of America, 85 24
-
T. Triglia, M. Peterson, D. Kemp (1988)
A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences.Nucleic acids research, 16 16
-
U. Gyllensten, H. Erlich (1988)
Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.Proceedings of the National Academy of Sciences of the United States of America, 85 20
-
(1989)
Interfacing in vitro DNA amplification with DNA sequencing strategies -
H. Ochman, Anne Gerber, D. Hart (1988)
Genetic applications of an inverse polymerase chain reaction.Genetics, 120 3
-
(1987)
Automated DNA sequence analysis -
L. Mcbride, C. Mccollum, S. Davidson, J. Efcavitch, A. Andrus, S. Lombardi (1988)
A new, reliable cartridge for the rapid purification of synthetic DNA.BioTechniques, 6 4
-
L. Hood, M. Hunkapiller, L. Smith (1987)
Automated DNA sequencing and analysis of the human genome.Genomics, 1 3
-
E. Stoflet, D. Koeberl, G. Sarkar, S. Sommer (1988)
Genomic amplification with transcript sequencing.Science, 239 4839
-
R. Wilson, A. Yuen, S. Clark, C. Spence, P. Arakelian, L. Hood (1988)
Automation of dideoxynucleotide DNA sequencing reactions using a robotic workstation.BioTechniques, 6 8
-
(1986)
Method of detecting electrophoretically separated oligonucleotides -
F. Sanger, S. Nicklen, A. Coulson (1977)
DNA sequencing with chain-terminating inhibitors.Proceedings of the National Academy of Sciences of the United States of America, 74 12
-
M. Caruthers, A. Barone, S. Beaucage, D. Dodds, E. Fisher, L. Mcbride, M. Matteucci, Z. Stabinsky, J. Tang (1987)
Chemical synthesis of deoxyoligonucleotides by the phosphoramidite method.Methods in enzymology, 154
-
K. Mullis, F. Faloona (1987)
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.Methods in enzymology, 155
-
(1987)
Chemical synthesis of deoxyoligonucleotides by the phosphoramidite approach
- Publisher
- Oxford University Press
- Copyright
- © 1989 The American Association for Clinical Chemistry, Inc.
- ISSN
- 0009-9147
- eISSN
- 1530-8561
- DOI
- 10.1093/clinchem/35.11.2196
- Publisher site
- See Article on Publisher Site
Abstract
Abstract Polymerase chain reaction (PCR) as a method for preparing DNA templates has been used for several DNA sequencing applications. An in situ procedure for directly sequencing PCR products by the dideoxy-termination method has been developed by using fluorophore-labeled sequencing primers. Completed sequence reactions were combined and loaded into a single electrophoretic lane of a fluorescence-based DNA sequence analyzer. DNA targets devoid of a universal primer sequence could be sequenced with labeled universal primers by incorporating a universal primer sequence into the PCR product. With this method, the sequence of a 351-bp region in the bacteriophage lambda genome was fully analyzed in a single lane with automatic base identification accuracy of greater than 99%. An unknown sequence, 1.7 kb long, also was sequenced by this procedure, in combination with a "PCR gene walking" strategy. Comparison of the 1110 bases in overlapping sequence data from both strands yielded only two single-base ambiguities. Automated DNA sequence analysis of the highly polymorphic HLA-DQA-1 (alpha) region in the human genome can be performed with this simple methodology. Use of this PCR-sequencing method to analyze DNA extracted from a one-month-old blood sample from an individual who is heterozygous at this locus allowed unambiguous assignment of genotype. This content is only available as a PDF. © 1989 The American Association for Clinical Chemistry, Inc. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
Journal
Clinical Chemistry – Oxford University Press
Published: Nov 1, 1989