Folia Microbiologica

Styk, B.; Závada, J.; Hána, L.

doi: 10.1007/bf02873304pmid: 14208476

The role of the “antibody cofactor” and of other heat-labile serum components (complement) in the neutralization of the ϕX 174 bacteriophage by means of specific antibodies was studied. Sera of white mice, guinea-pigs and rabbits obtained mainly early after phage administration were investigated. The character of antibodies was estimated from their sensitivity to 2-mercaptoethanol or else by chromatography on Sephadex G-200. Sera from the first days after the administration of the phage containing mostly type 19S antibodies, and sera from later periods after the administration containing mostly type 7S antibodies, were tested. (Some evidence was also obtained about the formation of slowly sedimenting antibodies sensitive to 2-mercaptoethanol in the rabbit.) With a single exception the tested sera showed no significant decrease of the neutralization activity after 30-mins. heating at 56°C or at 60°C and no increase of the neutralization power could be observed after the application of homologous or normal mouse serum. It is concluded that the heat-labile components of normal sera including the complement and the “antibody cofactor” play no role in specific phage neutralization.

Říha, I.

doi: 10.1007/bf02873309pmid: 14208481

It appears that anti-gamma-globulin sera not only affect the haemagglutinating reaction but can also be employed for detecting antibodies at the erythrocyte surface through the haemolytic reaction. The observed increase in the haemagglutinating as well as haemolytic reaction brought about by anti-gamma-globulin sera is due mainly to the effect on the reaction of 7 S anti-erythrocyte antibodies, the reaction of macroglobulin antibodies being little affected.