Sensitivity of Takifugu TRPA1 to thermal stimulations analyzed in oocytes expression system

Sensitivity of Takifugu TRPA1 to thermal stimulations analyzed in oocytes expression system Transient receptor potential ankyrin 1 (TRPA1s) from insects and several tetrapod vertebrates except rodents have been reported to be activated by high temperature with a relatively clear threshold. Our previous report, however, indicated that oocytes expressing zebrafish TRPA1b showed cold and heat-induced responses. Further, we also observed that zebrafish TRPA1b was gradually heat activated without a clear threshold. To study the possibility that these unique properties of thermal sensing of zebrafish TRPA1b are common to TRPA1s from other fish, we focused on the thermal response of pufferfish transient receptor potential ankyrin 1 (pfTRPA1) in the present study. By the two-electrode voltage clamp method using the Xenopus oocyte expression system, ionic currents were analyzed. Although some batches of pfTRPA1-expressing oocytes responded only to cold or heat stimulation, dual sensitivity to both cold and heat stimuli associated with pfTRPA1 expression was repeatedly observed. We detected a rapid response of oocytes expressing pfTRPA1 below 10°C and also a gradual activation above 25°C without an apparent threshold. The results indicated that these thermal sensitivities of fish TRPA1 are clearly different from those of TRPA1s of tetrapod vertebrates. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neuroreport Wolters Kluwer Health

Sensitivity of Takifugu TRPA1 to thermal stimulations analyzed in oocytes expression system

Loading next page...
 
/lp/wolters_kluwer/sensitivity-of-takifugu-trpa1-to-thermal-stimulations-analyzed-in-iHj2WFtX7y
Publisher
Wolters Kluwer Health
Copyright
Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.
ISSN
0959-4965
eISSN
1473-558X
D.O.I.
10.1097/WNR.0000000000000939
Publisher site
See Article on Publisher Site

Abstract

Transient receptor potential ankyrin 1 (TRPA1s) from insects and several tetrapod vertebrates except rodents have been reported to be activated by high temperature with a relatively clear threshold. Our previous report, however, indicated that oocytes expressing zebrafish TRPA1b showed cold and heat-induced responses. Further, we also observed that zebrafish TRPA1b was gradually heat activated without a clear threshold. To study the possibility that these unique properties of thermal sensing of zebrafish TRPA1b are common to TRPA1s from other fish, we focused on the thermal response of pufferfish transient receptor potential ankyrin 1 (pfTRPA1) in the present study. By the two-electrode voltage clamp method using the Xenopus oocyte expression system, ionic currents were analyzed. Although some batches of pfTRPA1-expressing oocytes responded only to cold or heat stimulation, dual sensitivity to both cold and heat stimuli associated with pfTRPA1 expression was repeatedly observed. We detected a rapid response of oocytes expressing pfTRPA1 below 10°C and also a gradual activation above 25°C without an apparent threshold. The results indicated that these thermal sensitivities of fish TRPA1 are clearly different from those of TRPA1s of tetrapod vertebrates.

Journal

NeuroreportWolters Kluwer Health

Published: Mar 7, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off