Calcium-regulated chloride channel anoctamin-1 is present in the suprachiasmatic nuclei of rats

Calcium-regulated chloride channel anoctamin-1 is present in the suprachiasmatic nuclei of rats Calcium-regulated chloride channel (CaCC) anoctamin-1 has been recently identified in neurons. In neurons, which express the Na+–K+–2Cl− cotransporter, activation of CaCCs increases firing frequency, by reversion between the Cl− equilibrium potential and the membrane resting potential, leading to membrane depolarization by Cl− extrusion from the cell. Although there are no reports of CaCCs present in the suprachiasmatic nuclei (SCN), the fact that Na+–K+–2Cl− cotransporter is present in SCN neurons, where it has been shown to be involved in the excitatory effects of γ-aminobutyric acid, together with the increase of neuronal firing rate induced by release of intracellular Ca2+ after administration of 100 nM ryanodine, leads us to determine whether CaCCs are present in the SCN. Immunohistochemistry and western blots show the expression of the CaCCs anoctamin-1 protein. Quantitative PCR demonstrated the expression of anoctamin-1 mRNA in the SCN. These results clearly indicate the presence of CaCC in SCN of rats. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neuroreport Wolters Kluwer Health

Calcium-regulated chloride channel anoctamin-1 is present in the suprachiasmatic nuclei of rats

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Publisher
Wolters Kluwer Health
Copyright
Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.
ISSN
0959-4965
eISSN
1473-558X
D.O.I.
10.1097/WNR.0000000000000967
Publisher site
See Article on Publisher Site

Abstract

Calcium-regulated chloride channel (CaCC) anoctamin-1 has been recently identified in neurons. In neurons, which express the Na+–K+–2Cl− cotransporter, activation of CaCCs increases firing frequency, by reversion between the Cl− equilibrium potential and the membrane resting potential, leading to membrane depolarization by Cl− extrusion from the cell. Although there are no reports of CaCCs present in the suprachiasmatic nuclei (SCN), the fact that Na+–K+–2Cl− cotransporter is present in SCN neurons, where it has been shown to be involved in the excitatory effects of γ-aminobutyric acid, together with the increase of neuronal firing rate induced by release of intracellular Ca2+ after administration of 100 nM ryanodine, leads us to determine whether CaCCs are present in the SCN. Immunohistochemistry and western blots show the expression of the CaCCs anoctamin-1 protein. Quantitative PCR demonstrated the expression of anoctamin-1 mRNA in the SCN. These results clearly indicate the presence of CaCC in SCN of rats.

Journal

NeuroreportWolters Kluwer Health

Published: Mar 7, 2018

References

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