Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Regulation of Vascular Smooth Muscle Cell Growth by Endothelial‐Synthesized Extracellular Matrices

Regulation of Vascular Smooth Muscle Cell Growth by Endothelial‐Synthesized Extracellular... Previous work has demonstrated that aortic endothelial cells (EC) produce a heparin-like inhibitor of smooth muscle cell (SMC) growth when both cell types were cultured on plastic. We have now tested the influence of the extracellular matrix on this EC-SMC interaction. Specifically, we examined: 1) the role of different substrates (plastic, fibronectin, monomeric, and fibrillar collagens I and Ill, and EC-derived matrices) on the growth rate and population density of SMC; 2) the heparin-sensitivity of SMC on these diverse substrates; and 3) the effect of these same substrates on EC ability to secrete heparin-like and polypeptide inhibitors of SMC growth. SMC demonstrated a sixfold difference in sensitivity to heparin when grown on different substrates, with the following rank order: EGTA matrix > collagens = plastic = fibronectin > deoxycholic acid (DOC) matrix. Maximally, we found a 10-fold difference in the potency of the inhibitory activity secreted by EC grown on different substrates, with the following order: plastic = EGTA matrix > fibronectin > collagens = DOC matrix. Treatment of the conditioned mediums with heparinase and trypsin indicated that 58% to 76% of the inhibitory activity was due to heparin-like species, and 24% to 42% was due to protein@). When EC cultured on EGTA matrix are compared to those plated on DOC matrix, the potency of the heparin-like and peptide inhibitory activities increased 8and 17-fold, respectively. Hypothetically, one would predict a 60-fold change in the potency of the antiproliferative effect if the contributions of substrate to EC production of inhibitors and SMC sensitivity were additive. In practice, we obtained 30to 40fold changes In potency between the different substrate combinations. These results indicate that EC-derived matrices strongly influence both the EC production of heparin-like and polypeptide inhibitory activity and the SMC response to the growth regulators. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arteriosclerosis Wolters Kluwer Health

Regulation of Vascular Smooth Muscle Cell Growth by Endothelial‐Synthesized Extracellular Matrices

Arteriosclerosis , Volume 7 (5) – Sep 1, 1987

Loading next page...
 
/lp/wolters-kluwer-health/regulation-of-vascular-smooth-muscle-cell-growth-by-endothelial-hyphen-01gjmT8CvR
Copyright
© 1987 by American Heart Association, Inc.
ISSN
1079-5642

Abstract

Previous work has demonstrated that aortic endothelial cells (EC) produce a heparin-like inhibitor of smooth muscle cell (SMC) growth when both cell types were cultured on plastic. We have now tested the influence of the extracellular matrix on this EC-SMC interaction. Specifically, we examined: 1) the role of different substrates (plastic, fibronectin, monomeric, and fibrillar collagens I and Ill, and EC-derived matrices) on the growth rate and population density of SMC; 2) the heparin-sensitivity of SMC on these diverse substrates; and 3) the effect of these same substrates on EC ability to secrete heparin-like and polypeptide inhibitors of SMC growth. SMC demonstrated a sixfold difference in sensitivity to heparin when grown on different substrates, with the following rank order: EGTA matrix > collagens = plastic = fibronectin > deoxycholic acid (DOC) matrix. Maximally, we found a 10-fold difference in the potency of the inhibitory activity secreted by EC grown on different substrates, with the following order: plastic = EGTA matrix > fibronectin > collagens = DOC matrix. Treatment of the conditioned mediums with heparinase and trypsin indicated that 58% to 76% of the inhibitory activity was due to heparin-like species, and 24% to 42% was due to protein@). When EC cultured on EGTA matrix are compared to those plated on DOC matrix, the potency of the heparin-like and peptide inhibitory activities increased 8and 17-fold, respectively. Hypothetically, one would predict a 60-fold change in the potency of the antiproliferative effect if the contributions of substrate to EC production of inhibitors and SMC sensitivity were additive. In practice, we obtained 30to 40fold changes In potency between the different substrate combinations. These results indicate that EC-derived matrices strongly influence both the EC production of heparin-like and polypeptide inhibitory activity and the SMC response to the growth regulators.

Journal

ArteriosclerosisWolters Kluwer Health

Published: Sep 1, 1987

There are no references for this article.