Expression of Apolipoprotein B Epitopes in Very Low Density Lipoprotein Subtractions
Expression of Apolipoprotein B Epitopes in Very Low Density Lipoprotein Subtractions
Tikkanen, Matti J.; Cole, Thomas G.; Hahm, Kyung-Soo ; Krul, Elaine S.; Schonfeld, Gustav
1984-03-01 00:00:00
Epitope expression was studied in both denatured apolipoprotein B (apo B) on Western blots and in intact low density lipoprotein (LDL) and very low density lipoprotein subtractions VLDL1(Sf120-400), VLDL2(Sf60-120), and VLDL3(S120-60) in competitive binding immunoassays with the aid of six monoclonal anti-LDL antibodies. The apo B in all lipoprotein fractions was shown to bind to all antibodies, but significant differences in apo B epitope expression were observed. On the average, the immunoreactivity of VLDL subtractions (expresssed as binding affinity and as relative 125I-LDL displacing potency) decreased with increasing flotation rate. Similarly, VLDL, was less immunoreactive than lipolyzed “remnants” of VLDL, after treatment with bovine milk lipoprotein lipase. The results indicate that, even when lipoprotein fractions obtained from the same individual and having the same kind of apo B subspecies were compared, significant differences in immunoreactivity occurred due to the modulating effect of other lipoprotein components on apo B epitope expression.
http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.pngArteriosclerosisWolters Kluwer Healthhttp://www.deepdyve.com/lp/wolters-kluwer-health/expression-of-apolipoprotein-b-epitopes-in-very-low-density-br7ercqhVj
Expression of Apolipoprotein B Epitopes in Very Low Density Lipoprotein Subtractions
Epitope expression was studied in both denatured apolipoprotein B (apo B) on Western blots and in intact low density lipoprotein (LDL) and very low density lipoprotein subtractions VLDL1(Sf120-400), VLDL2(Sf60-120), and VLDL3(S120-60) in competitive binding immunoassays with the aid of six monoclonal anti-LDL antibodies. The apo B in all lipoprotein fractions was shown to bind to all antibodies, but significant differences in apo B epitope expression were observed. On the average, the immunoreactivity of VLDL subtractions (expresssed as binding affinity and as relative 125I-LDL displacing potency) decreased with increasing flotation rate. Similarly, VLDL, was less immunoreactive than lipolyzed “remnants” of VLDL, after treatment with bovine milk lipoprotein lipase. The results indicate that, even when lipoprotein fractions obtained from the same individual and having the same kind of apo B subspecies were compared, significant differences in immunoreactivity occurred due to the modulating effect of other lipoprotein components on apo B epitope expression.
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