Zinc Uptake into Synaptosomes

Zinc Uptake into Synaptosomes Zinc uptake‘was studied in synaptosomes, isolated by the Ficoll flotation technique, using the radiotracer 65Zn. True uptake of zinc could be discriminated from binding to the outside of the synaptosomes by the absence of accumulation at 0°C and the dependency of the rate of uptake on the medium osmolarity. The zinc uptake, studied in the presence of various zinc‐complexing agents, showed saturation kinetics when analyzed in terms of (Zn)free, yielding Km= 0.25 μM. The zinc uptake was independent of both ATP and the Na+ gradient. No efflux of zinc could be demonstrated from preloaded synaptosomes due to the formation of insoluble zinc complexes inside the synaptosomes. The results are discussed in terms of the modulation of diverse neurochemical processes by zinc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

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Publisher
Wiley
Copyright
Copyright © 1988 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
D.O.I.
10.1111/j.1471-4159.1988.tb02982.x
Publisher site
See Article on Publisher Site

Abstract

Zinc uptake‘was studied in synaptosomes, isolated by the Ficoll flotation technique, using the radiotracer 65Zn. True uptake of zinc could be discriminated from binding to the outside of the synaptosomes by the absence of accumulation at 0°C and the dependency of the rate of uptake on the medium osmolarity. The zinc uptake, studied in the presence of various zinc‐complexing agents, showed saturation kinetics when analyzed in terms of (Zn)free, yielding Km= 0.25 μM. The zinc uptake was independent of both ATP and the Na+ gradient. No efflux of zinc could be demonstrated from preloaded synaptosomes due to the formation of insoluble zinc complexes inside the synaptosomes. The results are discussed in terms of the modulation of diverse neurochemical processes by zinc.

Journal

Journal of NeurochemistryWiley

Published: Mar 1, 1988

References

  • Free mitochondria and synaptosomes from single rat forebrain. A comparison between two known subfractionation techniques
    Dagani, Dagani; Zanada, Zanada; Marzatico, Marzatico; Benzi, Benzi
  • Optimized atomic absorption spectrophotometry of zinc in cerebrospinal fluid
    Palm, Palm; Sjöström, Sjöström; Hallmans, Hallmans
  • Kinetics of zinc uptake and exchange by primary cultures of rat hepatocytes
    Pattison, Pattison; Cousins, Cousins
  • Copper transport kinetics by isolated rat hepatocytes
    Schmitt, Schmitt; Darwish, Darwish; Cheney, Cheney; Ettinger, Ettinger

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