The CD34 molecule is a cell surface marker widely used to both identify and isolate hematopoietic progenitor cells. Although this marker was initially identified as an antigen expressed in progenitors cells, it has also been used to detect CD34+ leukemic cells, vascular endothelial cells, muscle satellite cells, and epidermal precursors . Flow cytometry is a rapid and reproducible method to quantify the numbers of circulating CD34+ cells following stem cell mobilization with cytokines, as well as to predict the total number of CD34+ harvested cells in leukapheresis products. Nowadays, CD34+ cell enumeration is routinely used in clinical transplantation centers to optimize stem cell collections for reconstituting the hematopoietic system following myeloablative therapies .First flow cytometry assays for CD34+ cell enumeration used indirect immunofluorescence techniques and red‐cell lysing procedures with centrifugation and washing steps. CD34+ counts were initially obtained using a dual‐platform technique, also known as the Milan protocol. Since then, different methodologies have been described for improved detection of CD34+ cells. Using mutiparametric flow cytometry and, in agreement with the International Society of Hematotherapy and Graft Engineering (ISHAGE), CD34+ cells are counted in combination with CD45 staining to eliminate nonleukocytes and debris . Current flow cytometry‐based methods for human
Cytometry – Wiley
Published: Jan 1, 2018
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