Hepatic microsomal biotransformation reactions with xenobiotic and steroid substrates have been investigated in 4‐non‐ylphenol (NP; 1, 5, 25, and 125 mg/kg body weight)‐treated juvenile Atlantic salmon (Salmo salar), in addition to control and estradiol‐17β (5 mg/kg, positive control)‐treated fish. Treatment of juvenile salmon with NP caused an initial increase and an apparent dose‐dependent decrease in progesterone 6β‐, 16α, and 17α‐hydroxylase activities in liver microsomes. 7‐Ethoxyresorufin O‐deethylase and UDP‐glucuronosyltransferase activities were also reduced. Plasma levels of estradiol‐17β (E2) were lowered 24–43% as a result of NP treatment. Immunochemical analysis of CYP1A, CYP2K‐like, and CYP3A‐like proteins showed 18%, 47%, and 30% reductions in enzyme‐linked immunosorbent assay absorbance levels, respectively, in the groups treated with 125 mg NP/kg fish. The group treated with E2 also showed similar reductions. In summary, the present study has demonstrated variations in steroid hydroxylases, cytochrome P450 isozymes, and conjugating enzyme levels in NP‐treated juvenile salmon. These results represent a novel aspect of NP effects not previously demonstrated with an environmental estrogen in any fish species or lower vertebrate.
Environmental Toxicology & Chemistry – Wiley
Published: Dec 1, 1997
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