Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You and Your Team.

Learn More →

Volume‐sensitive chloride channels in mouse cortical neurons: characterization and role in volume regulation

Volume‐sensitive chloride channels in mouse cortical neurons: characterization and role in volume... Because persistent swelling causes cell damage and often results in cell death, volume regulation is an important physiological function in both neuronal and non‐neuronal cells. Brain cell swelling has been observed not only in various pathological conditions but also during physiological synaptic transmissions. Volume‐sensitive anion channels have been reported to play an important role in the regulatory volume decrease occurring after osmotic swelling in many cell types. In this study, using a two‐photon laser scanning microscope and patch‐clamp techniques, we found that mouse cortical neurons in primary culture exhibit regulatory volume decrease after transient swelling and activation of Cl– currents during exposure to a hypotonic solution. The regulatory volume decrease was inhibited by Cl– channel blockers or K+ channel blockers. Swelling‐activated Cl– currents exhibited outward rectification, time‐dependent inactivation at large positive potentials, a low‐field anion permeability sequence, an intermediate unitary conductance and sensitivity to known blockers of volume‐sensitive Cl– channels. Thus, it is concluded that the activity of the volume‐sensitive outwardly rectifying Cl– channel plays a role in the control of cell volume in cortical neurons. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Neuroscience Wiley

Volume‐sensitive chloride channels in mouse cortical neurons: characterization and role in volume regulation

Download PDF
11 pages
Read Article

Loading next page...
 
/lp/wiley/volume-sensitive-chloride-channels-in-mouse-cortical-neurons-XN2HAfC8rK
Publisher
Wiley
Copyright
Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0953-816X
eISSN
1460-9568
DOI
10.1111/j.1460-9568.2005.04006.x
pmid
15845092
Publisher site
See Article on Publisher Site

Abstract

Because persistent swelling causes cell damage and often results in cell death, volume regulation is an important physiological function in both neuronal and non‐neuronal cells. Brain cell swelling has been observed not only in various pathological conditions but also during physiological synaptic transmissions. Volume‐sensitive anion channels have been reported to play an important role in the regulatory volume decrease occurring after osmotic swelling in many cell types. In this study, using a two‐photon laser scanning microscope and patch‐clamp techniques, we found that mouse cortical neurons in primary culture exhibit regulatory volume decrease after transient swelling and activation of Cl– currents during exposure to a hypotonic solution. The regulatory volume decrease was inhibited by Cl– channel blockers or K+ channel blockers. Swelling‐activated Cl– currents exhibited outward rectification, time‐dependent inactivation at large positive potentials, a low‐field anion permeability sequence, an intermediate unitary conductance and sensitivity to known blockers of volume‐sensitive Cl– channels. Thus, it is concluded that the activity of the volume‐sensitive outwardly rectifying Cl– channel plays a role in the control of cell volume in cortical neurons.

Journal

European Journal of Neuroscience – Wiley

Published: Mar 1, 2005

References

  • Volume changes induced by osmotic stress in freshly isolated rat hippocampal neurons
    Aitken, Aitken; Borgdorff, Borgdorff; Juta, Juta; Kiehart, Kiehart; Somjen, Somjen; Wadman, Wadman
  • Evidence against volume regulation by cortical brain cells during acute osmotic stress
    Andrew, Andrew; Lobinowich, Lobinowich; Osehobo, Osehobo
  • Imaging cell volume changes and neuronal excitation in the hippocampal slice
    Andrew, Andrew; MacVicar, MacVicar
  • Modulation of endogenous firing patterns by osmolarity in rat hippocampal neurones
    Azouz, Azouz; Alroy, Alroy; Yaari, Yaari
  • Responses of growth cones to changes in osmolality of the surrounding medium
    Bray, Bray; Money, Money; Harold, Harold; Bamburg, Bamburg
  • Induction of long‐term potentiation is associated with major ultrastructural changes of activated synapses
    Buchs, Buchs; Muller, Muller
  • Swelling‐ and cAMP‐activated Cl – currents in isolated rat carotid body type I cells
    Carpenter, Carpenter; Peers, Peers
  • Hypotonic exposure enhances synaptic transmission and triggers spreading depression in rat hippocampal tissue slices
    Chebabo, Chebabo; Hester, Hester; Aitken, Aitken; Somjen, Somjen
  • Cell cycle‐dependent expression of volume‐activated chloride currents in nasopharyngeal carcinoma cells
    Chen, Chen; Wang, Wang; Zhu, Zhu; Nie, Nie; Zhang, Zhang; Zhong, Zhong; Cai, Cai; Luo, Luo; Jacob, Jacob
  • Glutamate neurotoxicity in cortical cell culture
    Choi, Choi; Maulucci‐Gedde, Maulucci‐Gedde; Kriegstein, Kriegstein
  • NMDA receptor activation inhibits neuronal volume regulation after swelling induced by veratridine‐stimulated Na + influx in rat cortical cultures
    Churchwell, Churchwell; Wright, Wright; Emma, Emma; Rosenberg, Rosenberg; Strange, Strange
  • Transient decrease in water diffusion observed in human occipital cortex during visual stimulation
    Darquié, Darquié; Poline, Poline; Poupon, Poupon; Saint‐Jalmes, Saint‐Jalmes; Le Bihan, Le Bihan
  • A chloride channel reconstituted from fetal rat brain growth cones
    DeBin, DeBin; Wood, Wood; Pfenninger, Pfenninger; Strichartz, Strichartz
  • Osmolality‐induced changes in extracellular volume alter epileptiform bursts independent of chemical synapses in the rat: importance of non‐synaptic mechanisms in hippocampal epileptogenesis
    Dudek, Dudek; Obenaus, Obenaus; Tasker, Tasker
  • Dendritic spine changes associated with hippocampal long‐term synaptic plasticity
    Engert, Engert; Bonhoeffer, Bonhoeffer
  • Phloretin differentially inhibits volume‐sensitive and cyclic AMP‐activated, but not Ca 2+ ‐activated, Cl – channels
    Fan, Fan; Morishima, Morishima; Kida, Kida; Okada, Okada
  • Major differences in Ca 2+ i response to anoxia between neonatal and adult rat CA1 neurons: role of Ca 2+ o and Na + o
    Friedman, Friedman; Haddad, Haddad
  • Properties of a Cl – current activated by cell swelling in rabbit portal vein vascular smooth muscle cells
    Greenwood, Greenwood; Large, Large
  • Dendritic spines lost during glutamate receptor activation reemerge at original sites of synaptic contact
    Hasbani, Hasbani; Schlief, Schlief; Fisher, Fisher; Goldberg, Goldberg
  • Membrane mechanisms in volume and pH regulation in vertebrate cells
    Hoffmann, Hoffmann; Simonsen, Simonsen
  • Intrinsic optical signals in rat neocortical slices measured with near‐infrared dark‐field microscopy reveal changes in extracellular space
    Holthoff, Holthoff; Witte, Witte
  • Adaptation of cultured mammalian neurons to a hypotonic environment with age‐related response
    Horie, Horie; Ikuta, Ikuta; Takenaka, Takenaka; Ito, Ito
  • Activation of excitatory amino acid receptors in the rat hippocampal slice increases intracellular Cl – and cell
    Inglefield, Inglefield; Schwartz‐Bloom, Schwartz‐Bloom
  • Characterization of a fluorescence assay to monitor changes in the aqueous volume of lipid vesicles
    Kendall, Kendall; MacDonald, MacDonald
  • A high GluR1 : GluR2 expression ratio is correlated with expression of Ca 2+ ‐binding proteins in rat forebrain neurons
    Kondo, Kondo; Okabe, Okabe; Sumino, Sumino; Okado, Okado
  • Rapid and slow swelling during hypoxia in the CA1 region of rat hippocampal slices
    Kreisman, Kreisman; LaManna, LaManna
  • Taurine enhances volume regulation in hippocampal slices swollen osmotically
    Kreisman, Kreisman; Olson, Olson
  • Regional differences in hypoxic depolarization and swelling in hippocampal slices
    Kreisman, Kreisman; Soliman, Soliman; Gozal, Gozal
  • Functional significance of cell volume regulatory mechanisms
    Lang, Lang; Busch, Busch; Ritter, Ritter; Völkl, Völkl; Waldegger, Waldegger; Gulbins, Gulbins; Häussinger, Häussinger
  • A swelling‐activated chloride current in rat sympathetic neurones
    Leaney, Leaney; Marsh, Marsh; Brown, Brown
  • Experience‐dependent plasticity of dendritic spines in the developing rat barrel cortex in vivo
    Lendvai, Lendvai; Stern, Stern; Chen, Chen; Svoboda, Svoboda
  • Ionic changes and alterations in the size of the extracellular space during epileptic activity
    Lux, Lux; Heinemann, Heinemann; Dietzel, Dietzel
  • Normotonic cell shrinkage because of disordered volume regulation is an early prerequisite to apoptosis
    Maeno, Maeno; Ishizaki, Ishizaki; Kanaseki, Kanaseki; Hazama, Hazama; Okada, Okada
  • Inhibition of volume‐regulated anion channels in cultured endothelial cells by the anti‐oestrogens clomiphene and nafoxidine
    Maertens, Maertens; Droogmans, Droogmans; Chakraborty, Chakraborty; Nilius, Nilius
  • Rapid dendritic morphogenesis in CA1 hippocampal dendrites induced by synaptic activity
    Maletic‐Savatic, Maletic‐Savatic; Malinow, Malinow; Svoboda, Svoboda
  • Calcium, neuronal hyperexcitability and ischemic injury
    Meyer, Meyer
  • An increase in dendritic spine density on hippocampal CA1 pyramidal cells following spatial learning in adult rats suggests the formation of new synapses
    Moser, Moser; Trommald, Trommald; Andersen, Andersen
  • Effects of water on cortical excitability in humans
    Müller, Müller; Birbaumer, Birbaumer; Preißl, Preißl; Braun, Braun; Lang, Lang
  • Reversible loss of dendritic spines and altered excitability after chronic epilepsy in hippocampal slice cultures
    Müller, Müller; Gähwiler, Gähwiler; Rietschin, Rietschin; Thompson, Thompson
  • Modulation of the two‐pore domain acid‐sensitive K + channel TASK‐2 (KCNK5) by changes in cell
    Niemeyer, Niemeyer; Cid, Cid; Barros, Barros; Sepulveda, Sepulveda
  • Characterisation of a cell swelling‐activated K + ‐selective conductance of Ehrlich mouse ascites tumour cells
    Niemeyer, Niemeyer; Hougaard, Hougaard; Hoffmann, Hoffmann; Jorgensen, Jorgensen; Stutzin, Stutzin; Sepulveda, Sepulveda
  • Properties of volume‐regulated anion channels in mammalian cells
    Nilius, Nilius; Eggermont, Eggermont; Voets, Voets; Buyse, Buyse; Manolopoulos, Manolopoulos; Droogmans, Droogmans
  • Volume expansion‐sensing outward‐rectifier Cl – channel: fresh start to the molecular identity and volume sensor
    Okada, Okada
  • Volume‐regulatory ion channels in human epithelial cells
    Okada, Okada; Hazama, Hazama
  • Receptor‐mediated control of regulatory volume decrease (RVD) and apoptotic volume decrease (AVD)
    Okada, Okada; Maeno, Maeno; Shimizu, Shimizu; Dezaki, Dezaki; Wang, Wang; Morishima, Morishima
  • Dual roles of plasmalemmal chloride channels in induction of cell death
    Okada, Okada; Maeno, Maeno; Shimizu, Shimizu; Manabe, Manabe; Mori, Mori; Nabekura, Nabekura
  • Osmotic swelling activates intermediate‐conductance Cl – channels in human intestinal epithelial cells
    Okada, Okada; Petersen, Petersen; Kubo, Kubo; Morishima, Morishima; Tominaga, Tominaga
  • Mechanisms counteracting swelling in brain cells during hyponatremia
    Pasantes‐Morales, Pasantes‐Morales; Franco, Franco; Ordaz, Ordaz; Ochoa, Ochoa
  • Cell volume regulation in cultured cerebellar granule neurons
    Pasantes‐Morales, Pasantes‐Morales; Maar, Maar; Morán, Morán
  • Influence of calcium on regulatory volume decrease: role of potassium channels
    Pasantes‐Morales, Pasantes‐Morales; Morales‐Mulia, Morales‐Mulia
  • Disruption of mitochondrial respiration inhibits volume‐regulated anion channels and provokes neuronal cell swelling
    Patel, Patel; Lauritzen, Lauritzen; Lazdunski, Lazdunski; Honore, Honore
  • Lipid and mechano‐gated 2P domain K + channels
    Patel, Patel; Lazdunski, Lazdunski; Honore, Honore
  • Volume‐activated chloride currents contribute to the resting conductance and invasive migration of human glioma cells
    Ransom, Ransom; O'Neal, O'Neal; Sontheimer, Sontheimer
  • Cloning and expression of a novel pH‐sensitive two pore domain K + channel from human kidney
    Reyes, Reyes; Duprat, Duprat; Lesage, Lesage; Fink, Fink; Salinas, Salinas; Farman, Farman; Lazdunski, Lazdunski
  • Immunolocalization of the arachidonic acid and mechanosensitive baseline TAAK potassium channel in the nervous system
    Reyes, Reyes; Lauritzen, Lauritzen; Lesage, Lesage; Ettaiche, Ettaiche; Fosset, Fosset; Lazdunski, Lazdunski
  • The neurotoxicity of excitatory amino acids is produced by passive chloride influx
    Rothman, Rothman
  • K + channel block‐induced mammalian neuroblastoma cell swelling: a possible mechanism to influence proliferation
    Rouzaire‐Dubois, Rouzaire‐Dubois; Dubois, Dubois
  • Properties of K + and Cl – channels and their involvement in proliferation of rat microglial cells
    Schlichter, Schlichter; Sakellaropoulos, Sakellaropoulos; Ballyk, Ballyk; Pennefather, Pennefather; Phipps, Phipps
  • Differential expression of volume‐regulated anion channels during cell cycle progression of human cervical cancer cells
    Shen, Shen; Droogmans, Droogmans; Eggermont, Eggermont; Voets, Voets; Ellory, Ellory; Nilius, Nilius
  • A role of reactive oxygen species in apoptotic activation of volume‐sensitive Cl – channel
    Shimizu, Shimizu; Numata, Numata; Okada, Okada
  • Modulation of glioma cell migration and invasion using Cl – and K + ion channel blockers
    Soroceanu, Soroceanu; Manning, Manning; Sontheimer, Sontheimer
  • Pharmacologic properties of the swelling‐induced chloride current of dog atrial myocytes
    Sorota, Sorota
  • Cellular and molecular physiology of volume‐sensitive anion channels
    Strange, Strange; Emma, Emma; Jackson, Jackson
  • Glutamate injury‐induced epileptogenesis in hippocampal neurons: an in vitro model of stroke‐induced ‘epilepsy’
    Sun, Sun; Sombati, Sombati; DeLorenzo, DeLorenzo
  • GABAergic input contributes to activity‐dependent change in cell volume in the hippocampal CA1 region
    Takagi, Takagi; Obata, Obata; Tsubokawa, Tsubokawa
  • Swelling‐activated calcium signalling in cultured mouse primary sensory neurons
    Viana, Viana; De La Peña., De La Peña.; Pecson, Pecson; Schmidt, Schmidt; Belmonte, Belmonte
  • Blockers of volume‐activated Cl – currents inhibit endothelial cell proliferation
    Voets, Voets; Szucs, Szucs; Droogmans, Droogmans; Nilius, Nilius
  • Fluorescence techniques in microdetermination of metals in biological materials
    Wallach, Wallach; Steck, Steck
  • IK channels are involved in the regulatory volume decrease in human epithelial cells
    Wang, Wang; Morishima, Morishima; Okada, Okada
  • Blocking swelling‐activated chloride current inhibits mouse liver cell proliferation
    Wondergem, Wondergem; Gong, Gong; Monen, Monen; Dooley, Dooley; Gonce, Gonce; Conner, Conner; Houser, Houser; Ecay, Ecay; Ferslew, Ferslew
  • A volume‐sensitive chloride conductance in human colonic cell line T84
    Worrell, Worrell; Butt, Butt; Cliff, Cliff; Frizzell, Frizzell

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$499/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

Sign up
for free
Start 14 day
Free Trial