Vertical induction of engrailed‐2 and other region‐specific markers in the early chick embryo

Vertical induction of engrailed‐2 and other region‐specific markers in the early chick embryo We investigated the role of vertical signals in the regulation of Engrailed‐2, a regionally restricted (mesencephalon/metencephalon) neuroectodermal marker, using epiblast grafted from prospective neuroectoderm or prospective trunk mesoderm at mid‐stage 3 in the gastrulating chick embryo. Grafts that were isolated from the rostral (prospective neuroectodermal) epiblast and placed rostral to or at the future mesencephalon/metencephalon level, between the endoderm and epiblast of stage 3d to stage 8 host embryos, expressed Engrailed‐2 after 24 hr in culture, whereas these same grafts failed to express this marker when placed at a more caudal level. Grafts from caudal (prospective trunk mesodermal) epiblast, which would ordinarily not express Engrailed‐2, also expressed this marker when placed at the mesencephalon/metencephalon level, and failed to express it when grafted more caudally. The expression of four other markers, L5, Fgf8, Wnt‐1, and paraxis, were also evaluated. Collectively, our results show that regionally restricted vertical signals are capable of inducing neuroectoderm from naive tissue, and of patterning epiblast to express some but not all mesencephalon/metencephalon isthmus markers. Experiments using grafts taken from older embryos indicated that the competence of prospective neuroectoderm to become regionally patterned by vertical signals is gradually lost between stage 3c and stage 7. Similarly, prospective mesoderm from the caudal epiblast becomes unable to respond to vertical, neural‐inductive signals at these stages. These observations support a role for vertical signals in the induction and patterning of the neuroectoderm at gastrula and early neurula stages. Dev. Dyn. 209:45–58, 1997. © 1997 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Developmental Dynamics Wiley

Vertical induction of engrailed‐2 and other region‐specific markers in the early chick embryo

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Publisher
Wiley
Copyright
Copyright © 1997 Wiley‐Liss, Inc.
ISSN
1058-8388
eISSN
1097-0177
DOI
10.1002/(SICI)1097-0177(199705)209:1<45::AID-AJA5>3.0.CO;2-X
pmid
9142495
Publisher site
See Article on Publisher Site

Abstract

We investigated the role of vertical signals in the regulation of Engrailed‐2, a regionally restricted (mesencephalon/metencephalon) neuroectodermal marker, using epiblast grafted from prospective neuroectoderm or prospective trunk mesoderm at mid‐stage 3 in the gastrulating chick embryo. Grafts that were isolated from the rostral (prospective neuroectodermal) epiblast and placed rostral to or at the future mesencephalon/metencephalon level, between the endoderm and epiblast of stage 3d to stage 8 host embryos, expressed Engrailed‐2 after 24 hr in culture, whereas these same grafts failed to express this marker when placed at a more caudal level. Grafts from caudal (prospective trunk mesodermal) epiblast, which would ordinarily not express Engrailed‐2, also expressed this marker when placed at the mesencephalon/metencephalon level, and failed to express it when grafted more caudally. The expression of four other markers, L5, Fgf8, Wnt‐1, and paraxis, were also evaluated. Collectively, our results show that regionally restricted vertical signals are capable of inducing neuroectoderm from naive tissue, and of patterning epiblast to express some but not all mesencephalon/metencephalon isthmus markers. Experiments using grafts taken from older embryos indicated that the competence of prospective neuroectoderm to become regionally patterned by vertical signals is gradually lost between stage 3c and stage 7. Similarly, prospective mesoderm from the caudal epiblast becomes unable to respond to vertical, neural‐inductive signals at these stages. These observations support a role for vertical signals in the induction and patterning of the neuroectoderm at gastrula and early neurula stages. Dev. Dyn. 209:45–58, 1997. © 1997 Wiley‐Liss, Inc.

Journal

Developmental DynamicsWiley

Published: May 1, 1997

References

  • Determination events in the nervous system of the vertebrate embryo
    Bally‐Cuif, Bally‐Cuif; Wassef, Wassef
  • Changes in dorsoventral but not rostrocaudal regionalization of the chick neural tube in the absence of cranial notochord, as revealed by the expression of Engrailed‐2
    Darnell, Darnell; Schoenwolf, Schoenwolf; Ordahl, Ordahl
  • State of commitment of prospective neural plate and prospective mesoderm in late gastrula/early neurula stages of avian embryos
    Garcia‐Martinez, Garcia‐Martinez; Darnell, Darnell; Lopez‐Sanchez, Lopez‐Sanchez; Sosic, Sosic; Olson, Olson; Schoenwolf, Schoenwolf
  • Expression of an engrailed ‐like gene during development of the early embryonic chick nervous system
    Gardner, Gardner; Darnell, Darnell; Poole, Poole; Ordahl, Ordahl; Barald, Barald
  • Tracing neuroepithelial cells of the mesencephalic and metencephalic alar plates during cerebellar ontogeny in quail‐chick chimaeras
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    Millet, Millet; Alvarado‐Mallart, Alvarado‐Mallart
  • Localized BMP‐4 mediates dorsal/ventral patterning in the early Xenopus embryo
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    Xu, Xu; Kim, Kim; Taira, Taira; Zhan, Zhan; Sredni, Sredni; Kung, Kung
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