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Varied Transcriptional Efficiencies of Multiple Arabidopsis U6 Small Nuclear RNA Genes

Varied Transcriptional Efficiencies of Multiple Arabidopsis U6 Small Nuclear RNA Genes Arabidopsis U6 small nuclear RNA (snRNA) promoters are those transcribed by RNA polymerase III, but all the core elements for transcriptional initiation are located in the 5’promoter region. Previously, three Arabidopsis U6 snRNA genes (U6‐1, U6‐26, and U6‐29) were identified. Herein, we have further identified three new U6 loci (U6‐4, U6‐5, and U6‐6) in the Arabidopsis genome. Alignment of these revealed that the upstream sequence element and TATA elements were contained in six U6 promoters. In addition, a unique, highly conserved element named the “CAT” element was observed in the promoter region. To understand the expression patterns of these U6 genes in Arabidopsis, we fused these promoters to the DNA segment of β‐glucuronidase and then transferred these six constructs into Arabidopsis. Real‐time reverse transcription‐polymerase chain reaction analysis of these fused transcripts indicated that the newly identified U6 genes are active in Arabidopsis and that the U6‐26 promoter seems to have higher transcriptional activity in leaf, stem, flower and silique. These results help to understand the function of these U6 snRNAs in Arabidopsis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Integrative Plant Biology Wiley

Varied Transcriptional Efficiencies of Multiple Arabidopsis U6 Small Nuclear RNA Genes

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Publisher
Wiley
Copyright
Copyright © 2007 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1672-9072
eISSN
1744-7909
DOI
10.1111/j.1744-7909.2007.00393.x
Publisher site
See Article on Publisher Site

Abstract

Arabidopsis U6 small nuclear RNA (snRNA) promoters are those transcribed by RNA polymerase III, but all the core elements for transcriptional initiation are located in the 5’promoter region. Previously, three Arabidopsis U6 snRNA genes (U6‐1, U6‐26, and U6‐29) were identified. Herein, we have further identified three new U6 loci (U6‐4, U6‐5, and U6‐6) in the Arabidopsis genome. Alignment of these revealed that the upstream sequence element and TATA elements were contained in six U6 promoters. In addition, a unique, highly conserved element named the “CAT” element was observed in the promoter region. To understand the expression patterns of these U6 genes in Arabidopsis, we fused these promoters to the DNA segment of β‐glucuronidase and then transferred these six constructs into Arabidopsis. Real‐time reverse transcription‐polymerase chain reaction analysis of these fused transcripts indicated that the newly identified U6 genes are active in Arabidopsis and that the U6‐26 promoter seems to have higher transcriptional activity in leaf, stem, flower and silique. These results help to understand the function of these U6 snRNAs in Arabidopsis.

Journal

Journal of Integrative Plant Biology – Wiley

Published: Feb 1, 2007

Keywords: ; ; ; ;

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