Variable human minisatellite‐like regions in the Mycobacterium tuberculosis genome

Variable human minisatellite‐like regions in the Mycobacterium tuberculosis genome Mycobacterial interspersed repetitive units (MIRUs) are 40–100 bp DNA elements often found as tandem repeats and dispersed in intergenic regions of the Mycobacterium tuberculosis complex genomes. The M. tuberculosis H37Rv chromosome contains 41 MIRU loci. After polymerase chain reaction (PCR) and sequence analyses of these loci in 31 M. tuberculosis complex strains, 12 of them were found to display variations in tandem repeat copy numbers and, in most cases, sequence variations between repeat units as well. These features are reminiscent of those of certain human variable minisatellites. Of the 12 variable loci, only one was found to vary among genealogically distant BCG substrains, suggesting that these interspersed bacterial minisatellite‐like structures evolve slowly in mycobacterial populations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Microbiology Wiley

Variable human minisatellite‐like regions in the Mycobacterium tuberculosis genome

Loading next page...
 
/lp/wiley/variable-human-minisatellite-like-regions-in-the-mycobacterium-2ZCa1xAcKD
Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0950-382X
eISSN
1365-2958
DOI
10.1046/j.1365-2958.2000.01905.x
Publisher site
See Article on Publisher Site

Abstract

Mycobacterial interspersed repetitive units (MIRUs) are 40–100 bp DNA elements often found as tandem repeats and dispersed in intergenic regions of the Mycobacterium tuberculosis complex genomes. The M. tuberculosis H37Rv chromosome contains 41 MIRU loci. After polymerase chain reaction (PCR) and sequence analyses of these loci in 31 M. tuberculosis complex strains, 12 of them were found to display variations in tandem repeat copy numbers and, in most cases, sequence variations between repeat units as well. These features are reminiscent of those of certain human variable minisatellites. Of the 12 variable loci, only one was found to vary among genealogically distant BCG substrains, suggesting that these interspersed bacterial minisatellite‐like structures evolve slowly in mycobacterial populations.

Journal

Molecular MicrobiologyWiley

Published: May 1, 2000

References

  • Influences of array size and homogeneity on minisatellite mutation
    Buard, Buard; Bourdet, Bourdet; Yardley, Yardley; Dubrova, Dubrova; Jeffreys, Jeffreys
  • Identification of variable regions in the genomes of tubercle bacilli using bacterial artificial chromosome arrays
    Gordon, Gordon; Brosch, Brosch; Billault, Billault; Garnier, Garnier; Eiglmeier, Eiglmeier; Cole, Cole
  • Hypervariable digital DNA codes for human paternal lineages: MVR‐PCR at the Y‐specific minisatellite, MSY1 (DYF155S1)
    Jobling, Jobling; Bouzekri, Bouzekri; Taylor, Taylor
  • Y‐chromosome‐specific microsatellite mutation rates re‐examined using a minisatellite, MSY1
    Jobling, Jobling; Heyer, Heyer; Dieltjes, Dieltjes; De Knijf, De Knijf
  • DNA repair in Mycobacterium tuberculosis . What have we learnt from the genome sequence ?
    Mizrahi, Mizrahi; Andersen, Andersen
  • Characterization of the highly abundant polymorphic GC‐rich‐repetitive sequence (PGRS) present in Mycobacterium tuberculosis
    Poulet, Poulet; Cole, Cole
  • Identification of novel intergenic repetitive units in a mycobacterial two‐component system operon
    Supply, Supply; Magdalena, Magdalena; Himpens, Himpens; Locht, Locht

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off