Validation of an automated immune turbidimetric assay for serum gelsolin and its possible clinical utility in sepsis

Validation of an automated immune turbidimetric assay for serum gelsolin and its possible... INTRODUCTIONFor quick diagnosis and/or decision‐making when critically ill patients are considered, the need of fast and accurate laboratory methods for quantification of serum proteins with predictive value is of high importance. Human serum gelsolin (se‐GSN) is a Ca2+‐dependent 93 kDa protein mainly synthesized by skeletal muscle cells and consisting of 6 gelsolin homolog domains. Aside from its actin‐binding capacity, se‐GSN is suggested to be involved in the modulation of inflammatory processes, too. In severe systemic inflammation, as sepsis, se‐GSN levels drop significantly. Gelsolin is part of the so called actin scavenger system and therefore a possible reason for its decrease is the excessive release of filamentous actin into the circulation due to massive cell injury, and—in case of bacterial infections—the appearance of high amounts of bacterial wall lipids as well. Up to now, only a few studies have proven the possible predictive capacity of GSN levels in sepsis, severe burns, traumatic brain injury, but also in autoimmune diseases, chronic kidney disease, and HIV‐1 disease. However, even at present a rapid automated measurement of this actin‐binding protein still remains a challenge. The aim of the present work was to develop and validate a fast immune turbidimetric assay for serum GSN that http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Laboratory Analysis Wiley

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 Wiley Periodicals, Inc.
ISSN
0887-8013
eISSN
1098-2825
D.O.I.
10.1002/jcla.22321
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONFor quick diagnosis and/or decision‐making when critically ill patients are considered, the need of fast and accurate laboratory methods for quantification of serum proteins with predictive value is of high importance. Human serum gelsolin (se‐GSN) is a Ca2+‐dependent 93 kDa protein mainly synthesized by skeletal muscle cells and consisting of 6 gelsolin homolog domains. Aside from its actin‐binding capacity, se‐GSN is suggested to be involved in the modulation of inflammatory processes, too. In severe systemic inflammation, as sepsis, se‐GSN levels drop significantly. Gelsolin is part of the so called actin scavenger system and therefore a possible reason for its decrease is the excessive release of filamentous actin into the circulation due to massive cell injury, and—in case of bacterial infections—the appearance of high amounts of bacterial wall lipids as well. Up to now, only a few studies have proven the possible predictive capacity of GSN levels in sepsis, severe burns, traumatic brain injury, but also in autoimmune diseases, chronic kidney disease, and HIV‐1 disease. However, even at present a rapid automated measurement of this actin‐binding protein still remains a challenge. The aim of the present work was to develop and validate a fast immune turbidimetric assay for serum GSN that

Journal

Journal of Clinical Laboratory AnalysisWiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

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