Use of Bacillus subtilis PXN 21 spores for suppression of Clostridium difficile infection symptoms in a murine model

Use of Bacillus subtilis PXN 21 spores for suppression of Clostridium difficile infection... Clostridium difficile is the primary cause of nosocomial diarrhoea in healthcare centres of the developed world. Only a few antibiotics are available for treatment, and relapses are common in patients undergoing antibiotic therapy. New approaches are required to reduce reliance on antibiotics, the use of which represents a primary risk factor for development of C. difficile infections. Supplementation of the gut flora with probiotics represents a key area for producing more successful treatment options for C. difficile infection (CDI). In this study, spores of B. subtilis have been evaluated as a potential probiotic treatment against CDI. Using a murine model of infection, we demonstrate that oral administration of B. subtilis spores can attenuate the symptoms of infection. We further show that (1) suppression of symptoms was better if spores were administered post infection, and (2) germination of the spore to a vegetative cell may be an integral part of how CDI is suppressed. The results of this study highlight the potential of this bacterium as a probiotic treatment for CDI. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Fems Microbiology Letters Oxford University Press

Use of Bacillus subtilis PXN 21 spores for suppression of Clostridium difficile infection symptoms in a murine model

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Publisher
Oxford University Press
Copyright
Copyright © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd
ISSN
0378-1097
eISSN
1574-6968
D.O.I.
10.1111/1574-6968.12468
Publisher site
See Article on Publisher Site

Abstract

Clostridium difficile is the primary cause of nosocomial diarrhoea in healthcare centres of the developed world. Only a few antibiotics are available for treatment, and relapses are common in patients undergoing antibiotic therapy. New approaches are required to reduce reliance on antibiotics, the use of which represents a primary risk factor for development of C. difficile infections. Supplementation of the gut flora with probiotics represents a key area for producing more successful treatment options for C. difficile infection (CDI). In this study, spores of B. subtilis have been evaluated as a potential probiotic treatment against CDI. Using a murine model of infection, we demonstrate that oral administration of B. subtilis spores can attenuate the symptoms of infection. We further show that (1) suppression of symptoms was better if spores were administered post infection, and (2) germination of the spore to a vegetative cell may be an integral part of how CDI is suppressed. The results of this study highlight the potential of this bacterium as a probiotic treatment for CDI.

Journal

Fems Microbiology LettersOxford University Press

Published: Sep 1, 2014

References

  • Bacillus subtilis spores: a novel microparticle adjuvant which can instruct a balanced Th1 and Th2 immune response to specific antigen
    Barnes, Barnes; Cerovic, Cerovic; Hobson, Hobson; Klavinskis, Klavinskis
  • Immunostimulatory activity of Bacillus spores
    Huang, Huang; La Ragione, La Ragione; Nunez, Nunez; Cutting, Cutting
  • Faecal transplant for recurrent Clostridium difficile ‐associated diarrhoea: a UK case series
    MacConnachie, MacConnachie; Fox, Fox; Kennedy, Kennedy; Seaton, Seaton
  • Clostridium difficile ‐associated diarrhea: an emerging threat to pregnant women
    Rouphael, Rouphael; O'Donnell, O'Donnell; Bhatnagar, Bhatnagar

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