Uptake and Release of Tryptophan and Serotonin: An HPLC Method to Study the Flux of Endogenous 5‐Hydroxyindoles Through Synaptosomes

Uptake and Release of Tryptophan and Serotonin: An HPLC Method to Study the Flux of Endogenous... Abstract: An HPLC assay with fluorometric detection has been developed that is sensitive enough to measure simultaneously endogenous levels of tryptophan, serotonin (5‐hydroxytryptamine, or 5‐HT), and 5‐hydroxyindoleacetic acid (5‐HIAA) inside synaptosomes as well as that released into the incubation medium. Using this assay, we have observed that tryptophan is rapidly taken up by synaptosomes and turned over to 5‐HIAA without a concurrent release of 5‐HT. Exogenous 5‐HT is also rapidly taken up, and, within 20–30 min, 80% of the 5‐HT is deaminated. Veratridine induces release of both tryptophan and 5‐HT from synaptosomes. Changes in the disposition of exogenous tryptophan or 5‐HT can be completely accounted for by uptake or by stoichiometric changes in metabolites. This assay method should be valuable in the study of 5‐HT pools and in the determination of from which pool 5‐HT release occurs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Uptake and Release of Tryptophan and Serotonin: An HPLC Method to Study the Flux of Endogenous 5‐Hydroxyindoles Through Synaptosomes

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Publisher
Wiley
Copyright
Copyright © 1986 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1986.tb12925.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: An HPLC assay with fluorometric detection has been developed that is sensitive enough to measure simultaneously endogenous levels of tryptophan, serotonin (5‐hydroxytryptamine, or 5‐HT), and 5‐hydroxyindoleacetic acid (5‐HIAA) inside synaptosomes as well as that released into the incubation medium. Using this assay, we have observed that tryptophan is rapidly taken up by synaptosomes and turned over to 5‐HIAA without a concurrent release of 5‐HT. Exogenous 5‐HT is also rapidly taken up, and, within 20–30 min, 80% of the 5‐HT is deaminated. Veratridine induces release of both tryptophan and 5‐HT from synaptosomes. Changes in the disposition of exogenous tryptophan or 5‐HT can be completely accounted for by uptake or by stoichiometric changes in metabolites. This assay method should be valuable in the study of 5‐HT pools and in the determination of from which pool 5‐HT release occurs.

Journal

Journal of NeurochemistryWiley

Published: Jan 1, 1986

References

  • Characteristics of tryptophan accumulation by isolated rat forebrain synaptosomes
    Knowles, Knowles; Pogson, Pogson
  • Cation modulation of synaptosomal respiration
    Verity, Verity

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