Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types

Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types INTRODUCTIONHuman papillomavirus (HPV) is an established risk factor for invasive cervical cancer (ICC). It is well known that various HPV types infect the genital tract of many healthy young women, and persistent infection with certain HPV types deemed high risk induce cervical cancer. International prevalence studies have estimated that approximately 70% of ICCs are positive for HPV‐16 and ‐18, and approximately 27% of ICC involve the remaining high‐risk types or other types. HPV tests identifying HPV‐16 and ‐18 are valuable in screening for cervical cancer since those cases are at higher risk of malignant progression.There are at least 193 distinct molecular tests that are commercially available in the global market for the detection of HPV in cervical specimens. In studies using these HPV tests, a meta‐analysis showed that approximately 10% of cervical cancers tested negative for HPV. In many large‐scale epidemiological studies, PCR assays using the HPV L1 gene consensus primer sets, such as GP5+/GP6+, MY09/MY11, SPF‐10, and L1C1/L1C2 + L1C2M have been used. The advantage of these approaches is amplifying the L1 gene region for various HPV types in one reaction tube. However, these approaches have the disadvantage of lower sensitivity in unique HPV types with many mismatch sequences between http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Medical Virology Wiley

Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
© 2018 Wiley Periodicals, Inc.
ISSN
0146-6615
eISSN
1096-9071
D.O.I.
10.1002/jmv.25017
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONHuman papillomavirus (HPV) is an established risk factor for invasive cervical cancer (ICC). It is well known that various HPV types infect the genital tract of many healthy young women, and persistent infection with certain HPV types deemed high risk induce cervical cancer. International prevalence studies have estimated that approximately 70% of ICCs are positive for HPV‐16 and ‐18, and approximately 27% of ICC involve the remaining high‐risk types or other types. HPV tests identifying HPV‐16 and ‐18 are valuable in screening for cervical cancer since those cases are at higher risk of malignant progression.There are at least 193 distinct molecular tests that are commercially available in the global market for the detection of HPV in cervical specimens. In studies using these HPV tests, a meta‐analysis showed that approximately 10% of cervical cancers tested negative for HPV. In many large‐scale epidemiological studies, PCR assays using the HPV L1 gene consensus primer sets, such as GP5+/GP6+, MY09/MY11, SPF‐10, and L1C1/L1C2 + L1C2M have been used. The advantage of these approaches is amplifying the L1 gene region for various HPV types in one reaction tube. However, these approaches have the disadvantage of lower sensitivity in unique HPV types with many mismatch sequences between

Journal

Journal of Medical VirologyWiley

Published: Jan 1, 2018

Keywords: ; ;

References

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