Transplantation of cultured human adrenal chromaffin cells into 6‐hydroxydopamine‐lesioned rat brain

Transplantation of cultured human adrenal chromaffin cells into 6‐hydroxydopamine‐lesioned... Adult young rats were subjected to a unilateral 6‐hydroxydopamine lesion of the nigrostriatal dopamine pathway and then given intrastriatal grafts of human fetal adrenal chromaffin cell cultures. Amphetamine‐induced ipsiversive turning behavior in the lesioned rats was largely reversed in four of eight rats given such transplants when tested at 1.5 and 4.5 months post‐transplantation. Two rats showed a transient recovery at 1.5 months followed by deterioration at 4.5 months, while two other rats showed continuous deterioration. Six rats given sciatic nerve grafts as controls all showed deterioration from the pretransplantation levels. Catecholamine fluorescent and immunohistochemical examination of chromaffin‐cell‐transplanted brains demonstrated neurons and neuronal processes positive for catecholamines or tyrosine hydroxylase in the transplanted area. This transplantation of cultured human fetal cells to an animal model may provide the necessary basic experimental system for assessing the possible utility of human neuronal transplants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Synapse Wiley

Transplantation of cultured human adrenal chromaffin cells into 6‐hydroxydopamine‐lesioned rat brain

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Publisher
Wiley
Copyright
Copyright © 1987 Alan R. Liss, Inc.
ISSN
0887-4476
eISSN
1098-2396
D.O.I.
10.1002/syn.890010405
Publisher site
See Article on Publisher Site

Abstract

Adult young rats were subjected to a unilateral 6‐hydroxydopamine lesion of the nigrostriatal dopamine pathway and then given intrastriatal grafts of human fetal adrenal chromaffin cell cultures. Amphetamine‐induced ipsiversive turning behavior in the lesioned rats was largely reversed in four of eight rats given such transplants when tested at 1.5 and 4.5 months post‐transplantation. Two rats showed a transient recovery at 1.5 months followed by deterioration at 4.5 months, while two other rats showed continuous deterioration. Six rats given sciatic nerve grafts as controls all showed deterioration from the pretransplantation levels. Catecholamine fluorescent and immunohistochemical examination of chromaffin‐cell‐transplanted brains demonstrated neurons and neuronal processes positive for catecholamines or tyrosine hydroxylase in the transplanted area. This transplantation of cultured human fetal cells to an animal model may provide the necessary basic experimental system for assessing the possible utility of human neuronal transplants.

Journal

SynapseWiley

Published: Jan 1, 1987

References

  • Functional reinnervation of the neostriatum in the adult rat by use of intraparenchymal grafting of dissociated cell suspensions from the substantia nigra
    Bjöklund, Bjöklund; Schmidt, Schmidt; Stenevi, Stenevi
  • Immunocytochemical study of PC12 cells grafted to the brain of immature rats
    Jaeger, Jaeger
  • The origin and mechanism of the allograft reaction
    Laffery, Laffery; Woolnough, Woolnough
  • Functional activity of substantia nigra grafts reinnervating the striatum: Neurotransmitter metabolism and 14 C 2‐deoxy‐D‐glucose autoradiography
    Schmidt, Schmidt; Ingvar, Ingvar; Lindvall, Lindvall; Stenevi, Stenevi; Björklund, Björklund

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