Transplantation of artificial human lymphatic vascular tissues fabricated using a cell‐accumulation technique and their engraftment in mouse tissue with vascular remodelling

Transplantation of artificial human lymphatic vascular tissues fabricated using a... Transplantation of engineered tissues with microvascular structure is advancing towards therapeutic application to improve the flow of blood and/or lymphatic fluids. In lymphatic disorders, transplantation of tissue‐engineered lymphatic grafts can be an ideal treatment for draining excessive lymphatic fluid. In this study, we examined the transplantation of 3‐dimensional artificial human lymphatic network tissue (AHLT) fabricated by the cell accumulation technique into the subcutaneous tissue and fascia of mice. At 2 weeks after transplantation, the AHLT showed engraftment of artificial lymphatic vessels immunopositive for human CD31 and human podoplanin. Notably, we also observed the generation of blood vessel‐like structure comprising endothelial cells immunopositive for human CD34 and mural‐like cells immunopositive for human CD90 and αSMA, which were considered as myofibroblasts. In the fabrication of AHLT in vitro, the sporadic emergence of human CD34‐positive/Prox‐1‐negative sites was observed, followed by the formation of blood vessel‐like structure in the graft within 7 days after transplantation. The fine structure of engrafted AHLT observed by transmission electron microscopy showed that the engrafted artificial lymphatic vessels possess the specific structures of native lymphatic capillaries such as loose interendothelial connections and anchoring filaments. In contrast, blood vessel‐like structure showed tight interendothelial connections, thick basement membranes, and layers of mural‐like cells, which resemble small blood vessels. These results suggested the remodelling of artificial lymphatic network to form blood vessel‐like structure associated with mural‐like cells along with AHLT fabrication and engraftment. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Tissue Engineering and Regenerative Medicine Wiley

Transplantation of artificial human lymphatic vascular tissues fabricated using a cell‐accumulation technique and their engraftment in mouse tissue with vascular remodelling

Loading next page...
 
/lp/wiley/transplantation-of-artificial-human-lymphatic-vascular-tissues-N33f09nISr
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 John Wiley & Sons, Ltd.
ISSN
1932-6254
eISSN
1932-7005
D.O.I.
10.1002/term.2570
Publisher site
See Article on Publisher Site

Abstract

Transplantation of engineered tissues with microvascular structure is advancing towards therapeutic application to improve the flow of blood and/or lymphatic fluids. In lymphatic disorders, transplantation of tissue‐engineered lymphatic grafts can be an ideal treatment for draining excessive lymphatic fluid. In this study, we examined the transplantation of 3‐dimensional artificial human lymphatic network tissue (AHLT) fabricated by the cell accumulation technique into the subcutaneous tissue and fascia of mice. At 2 weeks after transplantation, the AHLT showed engraftment of artificial lymphatic vessels immunopositive for human CD31 and human podoplanin. Notably, we also observed the generation of blood vessel‐like structure comprising endothelial cells immunopositive for human CD34 and mural‐like cells immunopositive for human CD90 and αSMA, which were considered as myofibroblasts. In the fabrication of AHLT in vitro, the sporadic emergence of human CD34‐positive/Prox‐1‐negative sites was observed, followed by the formation of blood vessel‐like structure in the graft within 7 days after transplantation. The fine structure of engrafted AHLT observed by transmission electron microscopy showed that the engrafted artificial lymphatic vessels possess the specific structures of native lymphatic capillaries such as loose interendothelial connections and anchoring filaments. In contrast, blood vessel‐like structure showed tight interendothelial connections, thick basement membranes, and layers of mural‐like cells, which resemble small blood vessels. These results suggested the remodelling of artificial lymphatic network to form blood vessel‐like structure associated with mural‐like cells along with AHLT fabrication and engraftment.

Journal

Journal of Tissue Engineering and Regenerative MedicineWiley

Published: Jan 1, 2018

Keywords: ; ; ; ; ; ;

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial