Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Transactivation properties of parsley proline‐rich bZIP transcription factors

Transactivation properties of parsley proline‐rich bZIP transcription factors Light‐responsive chalcone synthase (CHS) gene activation requires LRUCHS, a light regulatory promoter unit including the MYB recognition element MRECHS and the ACGT‐containing element ACECHS. ACECHS is bound by the parsley basic region/leucine zipper (bZIP) factors CPRF1 and 4. Factors containing the bZIP domain exist in animals, plants and yeast, and recognize DNA sequence‐specifically after formation of homo‐ or heterodimers. To determine the potential role of CPRFs in the regulation of CHS promoter activity, we investigated the functions of distinct CPRF domains in a homologous co‐transfection system. The proline‐rich domains of CPRF1 and CPRF4 activate transcription, indicating that CPRF1 and CPRF4 have transactivating properties. Over‐expression of the CPRF1 bZIP domain caused a reduction of LRUCHS‐mediated light inducibility, and point mutations throughout ACECHS affected both responsiveness to UV‐containing white light and transactivation by CPRF1::VP16. The data suggest that a CPRF1‐containing bZIP heterodimer interacts with ACECHSin vivo. We discuss regulatory steps in light‐induced CHS transcription that may be influenced by CPRF1 and/or related bZIP factors. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

Transactivation properties of parsley proline‐rich bZIP transcription factors

Loading next page...
 
/lp/wiley/transactivation-properties-of-parsley-proline-rich-bzip-transcription-pRVBOV5JRc

References (36)

Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
DOI
10.1046/j.1365-313x.2000.00687.x
Publisher site
See Article on Publisher Site

Abstract

Light‐responsive chalcone synthase (CHS) gene activation requires LRUCHS, a light regulatory promoter unit including the MYB recognition element MRECHS and the ACGT‐containing element ACECHS. ACECHS is bound by the parsley basic region/leucine zipper (bZIP) factors CPRF1 and 4. Factors containing the bZIP domain exist in animals, plants and yeast, and recognize DNA sequence‐specifically after formation of homo‐ or heterodimers. To determine the potential role of CPRFs in the regulation of CHS promoter activity, we investigated the functions of distinct CPRF domains in a homologous co‐transfection system. The proline‐rich domains of CPRF1 and CPRF4 activate transcription, indicating that CPRF1 and CPRF4 have transactivating properties. Over‐expression of the CPRF1 bZIP domain caused a reduction of LRUCHS‐mediated light inducibility, and point mutations throughout ACECHS affected both responsiveness to UV‐containing white light and transactivation by CPRF1::VP16. The data suggest that a CPRF1‐containing bZIP heterodimer interacts with ACECHSin vivo. We discuss regulatory steps in light‐induced CHS transcription that may be influenced by CPRF1 and/or related bZIP factors.

Journal

The Plant JournalWiley

Published: Apr 1, 2000

There are no references for this article.