Tobacco retinoblastoma‐related protein phosphorylated by a distinct cyclin‐dependent kinase complex with Cdc2/cyclin D in vitro

Tobacco retinoblastoma‐related protein phosphorylated by a distinct cyclin‐dependent kinase... Summary The retinoblastoma (Rb) protein was originally identified as a product of a tumour suppressor gene that plays a pivotal role in regulating both the cell cycle and differentiation in mammals. The growth‐suppressive activity of Rb is regulated by phosphorylation with cyclin‐dependent kinase (CDK), and inactivation of the Rb function is one of the critical steps for transition from the G1 to the S phase. We report here the cloning of a cDNA (NtRb1) from Nicotiana tabacum which encodes a Rb‐related protein, and show that this gene is expressed in all the organs examined at the mRNA level. We have demonstrated that NtRb1 interacts with tobacco cyclin D by using yeast two‐hybrid and in vitro binding assays. In mammals, cyclin D can assemble with CDK4 and CDK6, but not with Cdc2, to form active complexes. Surprisingly, tobacco cyclin D and Cdc2 proteins can form a complex in insect cells, which is able to phosphorylate tobacco Rb‐related protein in vitro. Using immunoprecipitation with the anti‐cyclin D antibody, cyclin D can be found in a complex with Cdc2 in suspension‐cultured tobacco BY‐2 cells. These results suggest that the cdc2 gene modulates the cell cycle through the phosphorylation of Rb‐related protein by forming an active complex with cyclin D in plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

Tobacco retinoblastoma‐related protein phosphorylated by a distinct cyclin‐dependent kinase complex with Cdc2/cyclin D in vitro

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Publisher
Wiley
Copyright
Copyright © 1999 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
D.O.I.
10.1046/j.1365-313X.1999.00449.x
Publisher site
See Article on Publisher Site

Abstract

Summary The retinoblastoma (Rb) protein was originally identified as a product of a tumour suppressor gene that plays a pivotal role in regulating both the cell cycle and differentiation in mammals. The growth‐suppressive activity of Rb is regulated by phosphorylation with cyclin‐dependent kinase (CDK), and inactivation of the Rb function is one of the critical steps for transition from the G1 to the S phase. We report here the cloning of a cDNA (NtRb1) from Nicotiana tabacum which encodes a Rb‐related protein, and show that this gene is expressed in all the organs examined at the mRNA level. We have demonstrated that NtRb1 interacts with tobacco cyclin D by using yeast two‐hybrid and in vitro binding assays. In mammals, cyclin D can assemble with CDK4 and CDK6, but not with Cdc2, to form active complexes. Surprisingly, tobacco cyclin D and Cdc2 proteins can form a complex in insect cells, which is able to phosphorylate tobacco Rb‐related protein in vitro. Using immunoprecipitation with the anti‐cyclin D antibody, cyclin D can be found in a complex with Cdc2 in suspension‐cultured tobacco BY‐2 cells. These results suggest that the cdc2 gene modulates the cell cycle through the phosphorylation of Rb‐related protein by forming an active complex with cyclin D in plants.

Journal

The Plant JournalWiley

Published: May 1, 1999

References

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