The Use of Yeast Two‐Hybrid Screens in Studies of Protein:Protein Interactions Involved in Trafficking

The Use of Yeast Two‐Hybrid Screens in Studies of Protein:Protein Interactions Involved in... The yeast two‐hybrid system has provided a convenient means to both screen for proteins that interact with a protein of interest and to characterise the known interaction between two proteins. Several groups with an interest in the molecular mechanisms that underlie discrete steps along trafficking pathways have exploited the yeast two‐hybrid system. Here, we provide a brief background to the technology, attempt to point out some of the pitfalls and benefits of the different systems that can be employed, and mention some of the areas (within the trafficking field) where yeast two‐hybrid interaction assays have been particularly informative. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Traffic Wiley

The Use of Yeast Two‐Hybrid Screens in Studies of Protein:Protein Interactions Involved in Trafficking

Traffic, Volume 1 (10) – Oct 1, 2000

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Publisher
Wiley
Copyright
"Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company"
ISSN
1398-9219
eISSN
1600-0854
D.O.I.
10.1034/j.1600-0854.2000.011003.x
Publisher site
See Article on Publisher Site

Abstract

The yeast two‐hybrid system has provided a convenient means to both screen for proteins that interact with a protein of interest and to characterise the known interaction between two proteins. Several groups with an interest in the molecular mechanisms that underlie discrete steps along trafficking pathways have exploited the yeast two‐hybrid system. Here, we provide a brief background to the technology, attempt to point out some of the pitfalls and benefits of the different systems that can be employed, and mention some of the areas (within the trafficking field) where yeast two‐hybrid interaction assays have been particularly informative.

Journal

TrafficWiley

Published: Oct 1, 2000

Keywords: ; ;

References

  • Two hybrid system screen with the small GTP‐binding protein Rab6. Identification f a novel mouse GDP dissociation inhibitor and two other potential partners of Rab6
    Janoueix‐Lerosey, I; Jollivet, F; Camonis, J; Marche, PN; Goud, B

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