The theoretical reliability of PCR‐based fish viral diagnostic methods is critically affected when they are applied to fish populations with low prevalence and virus loads

The theoretical reliability of PCR‐based fish viral diagnostic methods is critically affected... IntroductionIn fish virology, diagnosis and its reliability is one of the main issues. Therefore, due to the growing relevance of fish viral diseases in aquaculture, many laboratories are introducing viral diagnostic techniques among their routine procedures. For most fish viruses, international organizations as the World Organization for Animal Health (OIE) or the European Union Reference Laboratory for Fish Diseases (EURL) are devoted to the designs and standardization of diagnostic procedures, which must be used for official purposes (OIE ; EURL ). Nevertheless, in most cases the laboratories may choose protocols developed by them or reported by other, often lacking the necessary objective quantitative information on their reliability. Frequently, the available information is limited to the analytical sensitivity, in terms of limit of detection (LOD), evaluated using raw virus as reference (Dopazo and Bandin ). This happens mainly with the most popular diagnostic technology nowadays: the polymerase chain reaction (PCR) and PCR‐derived techniques. PCR was first introduced as a molecular diagnostic technique for fish virology in the early 1990s, applied to both isolated and infectious fish tissues (Rimstad et al. 1990a; Blake et al. ) and, due to its higher sensitivity, it quickly displaced other molecular techniques that had been introduced shortly http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Microbiology Wiley

The theoretical reliability of PCR‐based fish viral diagnostic methods is critically affected when they are applied to fish populations with low prevalence and virus loads

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 The Society for Applied Microbiology
ISSN
1364-5072
eISSN
1365-2672
D.O.I.
10.1111/jam.13586
Publisher site
See Article on Publisher Site

Abstract

IntroductionIn fish virology, diagnosis and its reliability is one of the main issues. Therefore, due to the growing relevance of fish viral diseases in aquaculture, many laboratories are introducing viral diagnostic techniques among their routine procedures. For most fish viruses, international organizations as the World Organization for Animal Health (OIE) or the European Union Reference Laboratory for Fish Diseases (EURL) are devoted to the designs and standardization of diagnostic procedures, which must be used for official purposes (OIE ; EURL ). Nevertheless, in most cases the laboratories may choose protocols developed by them or reported by other, often lacking the necessary objective quantitative information on their reliability. Frequently, the available information is limited to the analytical sensitivity, in terms of limit of detection (LOD), evaluated using raw virus as reference (Dopazo and Bandin ). This happens mainly with the most popular diagnostic technology nowadays: the polymerase chain reaction (PCR) and PCR‐derived techniques. PCR was first introduced as a molecular diagnostic technique for fish virology in the early 1990s, applied to both isolated and infectious fish tissues (Rimstad et al. 1990a; Blake et al. ) and, due to its higher sensitivity, it quickly displaced other molecular techniques that had been introduced shortly

Journal

Journal of Applied MicrobiologyWiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

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