IntroductionIn fish virology, diagnosis and its reliability is one of the main issues. Therefore, due to the growing relevance of fish viral diseases in aquaculture, many laboratories are introducing viral diagnostic techniques among their routine procedures. For most fish viruses, international organizations as the World Organization for Animal Health (OIE) or the European Union Reference Laboratory for Fish Diseases (EURL) are devoted to the designs and standardization of diagnostic procedures, which must be used for official purposes (OIE ; EURL ). Nevertheless, in most cases the laboratories may choose protocols developed by them or reported by other, often lacking the necessary objective quantitative information on their reliability. Frequently, the available information is limited to the analytical sensitivity, in terms of limit of detection (LOD), evaluated using raw virus as reference (Dopazo and Bandin ). This happens mainly with the most popular diagnostic technology nowadays: the polymerase chain reaction (PCR) and PCR‐derived techniques. PCR was first introduced as a molecular diagnostic technique for fish virology in the early 1990s, applied to both isolated and infectious fish tissues (Rimstad et al. 1990a; Blake et al. ) and, due to its higher sensitivity, it quickly displaced other molecular techniques that had been introduced shortly
Journal of Applied Microbiology – Wiley
Published: Jan 1, 2018
Keywords: ; ; ; ;
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