The role of Helicobacter pylori DnaA domain I in orisome assembly on a bipartite origin of chromosome replication

The role of Helicobacter pylori DnaA domain I in orisome assembly on a bipartite origin of... The main roles of the DnaA protein are to bind the origin of chromosome replication (oriC), to unwind DNA and to provide a hub for the step‐wise assembly of a replisome. DnaA is composed of four domains, with each playing a distinct functional role in the orisome assembly. Out of the four domains, the role of domain I is the least understood and appears to be the most species‐specific. To better characterise Helicobacter pylori DnaA domain I, we have constructed a series of DnaA variants and studied their interactions with H. pylori bipartite oriC. We show that domain I is responsible for the stabilisation and organisation of DnaA‐oriC complexes and provides cooperativity in DnaA–DNA interactions. Domain I mediates cross‐interactions between oriC subcomplexes, which indicates that domain I is important for long‐distance DnaA interactions and is essential for orisosme assembly on bipartite origins. HobA, which interacts with domain I, increases the DnaA binding to bipartite oriC; however, it does not stimulate but rather inhibits DNA unwinding. This suggests that HobA helps DnaA to bind oriC, but an unknown factor triggers DNA unwinding. Together, our results indicate that domain I self‐interaction is important for the DnaA assembly on bipartite H. pylori oriC. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Microbiology Wiley

The role of Helicobacter pylori DnaA domain I in orisome assembly on a bipartite origin of chromosome replication

Loading next page...
 
/lp/wiley/the-role-of-helicobacter-pylori-dnaa-domain-i-in-orisome-assembly-on-a-xQ22gUmt2B
Publisher
Wiley
Copyright
Copyright © 2020 John Wiley & Sons Ltd
ISSN
0950-382X
eISSN
1365-2958
DOI
10.1111/mmi.14423
Publisher site
See Article on Publisher Site

Abstract

The main roles of the DnaA protein are to bind the origin of chromosome replication (oriC), to unwind DNA and to provide a hub for the step‐wise assembly of a replisome. DnaA is composed of four domains, with each playing a distinct functional role in the orisome assembly. Out of the four domains, the role of domain I is the least understood and appears to be the most species‐specific. To better characterise Helicobacter pylori DnaA domain I, we have constructed a series of DnaA variants and studied their interactions with H. pylori bipartite oriC. We show that domain I is responsible for the stabilisation and organisation of DnaA‐oriC complexes and provides cooperativity in DnaA–DNA interactions. Domain I mediates cross‐interactions between oriC subcomplexes, which indicates that domain I is important for long‐distance DnaA interactions and is essential for orisosme assembly on bipartite origins. HobA, which interacts with domain I, increases the DnaA binding to bipartite oriC; however, it does not stimulate but rather inhibits DNA unwinding. This suggests that HobA helps DnaA to bind oriC, but an unknown factor triggers DNA unwinding. Together, our results indicate that domain I self‐interaction is important for the DnaA assembly on bipartite H. pylori oriC.

Journal

Molecular MicrobiologyWiley

Published: Feb 1, 2020

Keywords: ; ; ; ;

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off