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The release of virus‐like particles from recombinant Saccharomyces cerevisiae : Effect of freezing and thawing on homogenization and bead milling

The release of virus‐like particles from recombinant Saccharomyces cerevisiae : Effect of... 10.1002/bit.260440610.abs Recombinant cells of Saccharomyces cerevisiae, expressing virus‐like particles (Ty‐VLPs), can be readily disrupted in a high pressure homogenizer and show identical disruption kinetics to the untransformed host strain. When the cells are freeze/thawed before disruption, they become about four times more resistant to homogenization. This effect increases with the number of freeze/thaw cycles, but is independent of the time the cells remain frozen. The freeze/thaw effect is observed with cells harvested during both the logarithmic and stationary phase of growth, and occurs with the untransformed host strain as well as the transformed one. Freeze/thawed cells are twice as resistant to disruption in the bead mill as fresh cells. © 1994 John Wiley & Sons, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology and Bioengineering Wiley

The release of virus‐like particles from recombinant Saccharomyces cerevisiae : Effect of freezing and thawing on homogenization and bead milling

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References (29)

Publisher
Wiley
Copyright
Copyright © 1994 John Wiley & Sons, Inc.
ISSN
0006-3592
eISSN
1097-0290
DOI
10.1002/bit.260440610
pmid
18618834
Publisher site
See Article on Publisher Site

Abstract

10.1002/bit.260440610.abs Recombinant cells of Saccharomyces cerevisiae, expressing virus‐like particles (Ty‐VLPs), can be readily disrupted in a high pressure homogenizer and show identical disruption kinetics to the untransformed host strain. When the cells are freeze/thawed before disruption, they become about four times more resistant to homogenization. This effect increases with the number of freeze/thaw cycles, but is independent of the time the cells remain frozen. The freeze/thaw effect is observed with cells harvested during both the logarithmic and stationary phase of growth, and occurs with the untransformed host strain as well as the transformed one. Freeze/thawed cells are twice as resistant to disruption in the bead mill as fresh cells. © 1994 John Wiley & Sons, Inc.

Journal

Biotechnology and BioengineeringWiley

Published: Sep 5, 1994

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