The Recovery and Persistence of Salivary DNA on Human Skin

The Recovery and Persistence of Salivary DNA on Human Skin Abstract: Salivary DNA is encountered in many crimes, such as sexual assaults and murders. In this study, saliva from three male donors was deposited on the skin of three female recipients. The amount of male salivary DNA remaining on the female skin was measured over a 96‐h period using the Quantifiler™ Y Human Male DNA Quantification Kit. In eight of the nine experiments, a full male DNA profile matching the donor was obtained even after 96 h. In addition, the study showed that the concentration of salivary DNA varied from donor to donor and from day to day. The efficiency of two recovery methods, wet and dry swabbing and minitaping, was compared. The results indicate the tapelift method gave higher DNA recovery. This study also examined the secondary transfer of salivary DNA from skin to fabrics. Cotton and polyester give higher DNA transfer than leather. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Forensic Sciences Wiley

The Recovery and Persistence of Salivary DNA on Human Skin

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Publisher
Wiley
Copyright
© 2010 American Academy of Forensic Sciences
ISSN
0022-1198
eISSN
1556-4029
D.O.I.
10.1111/j.1556-4029.2010.01520.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Salivary DNA is encountered in many crimes, such as sexual assaults and murders. In this study, saliva from three male donors was deposited on the skin of three female recipients. The amount of male salivary DNA remaining on the female skin was measured over a 96‐h period using the Quantifiler™ Y Human Male DNA Quantification Kit. In eight of the nine experiments, a full male DNA profile matching the donor was obtained even after 96 h. In addition, the study showed that the concentration of salivary DNA varied from donor to donor and from day to day. The efficiency of two recovery methods, wet and dry swabbing and minitaping, was compared. The results indicate the tapelift method gave higher DNA recovery. This study also examined the secondary transfer of salivary DNA from skin to fabrics. Cotton and polyester give higher DNA transfer than leather.

Journal

Journal of Forensic SciencesWiley

Published: Jan 1, 2011

References

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