The nitrogen response of a barley C‐hordein promoter is controlled by positive and negative regulation of the GCN4 and endosperm box

The nitrogen response of a barley C‐hordein promoter is controlled by positive and negative... The 431 bp C‐hordein promoter of λ‐1‐17 exhibits a specific response to amino acids and NH4NO3 in developing barley (Hordeum vulgare L.) endosperms. With the aid of particle bombardment it is shown that the GCN4 motif ATGA(C/G)TCAT is the dominating cis‐acting element in this response. But synergistic interaction with the neighbouring endosperm motif TGTAAAGT within the bifactorial prolamin element and cooperation with upstream sequences including a second prolamin‐like element is an absolute requirement for a strong, positive regulation by an optimal nitrogen regime. Low nitrogen levels convert the GCN4 box into a negative motif. In contrast the endosperm box on its own exerted a silencing activity, independent of nitrogen nutrition. Sequence comparisons revealed that GCN4‐ and endosperm‐like motifs are widely distributed among plant promoters. Their putative role in nitrogen regulation is discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

The nitrogen response of a barley C‐hordein promoter is controlled by positive and negative regulation of the GCN4 and endosperm box

The Plant Journal, Volume 4 (2) – Aug 1, 1993

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Publisher
Wiley
Copyright
Copyright © 1993 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
DOI
10.1046/j.1365-313X.1993.04020343.x
Publisher site
See Article on Publisher Site

Abstract

The 431 bp C‐hordein promoter of λ‐1‐17 exhibits a specific response to amino acids and NH4NO3 in developing barley (Hordeum vulgare L.) endosperms. With the aid of particle bombardment it is shown that the GCN4 motif ATGA(C/G)TCAT is the dominating cis‐acting element in this response. But synergistic interaction with the neighbouring endosperm motif TGTAAAGT within the bifactorial prolamin element and cooperation with upstream sequences including a second prolamin‐like element is an absolute requirement for a strong, positive regulation by an optimal nitrogen regime. Low nitrogen levels convert the GCN4 box into a negative motif. In contrast the endosperm box on its own exerted a silencing activity, independent of nitrogen nutrition. Sequence comparisons revealed that GCN4‐ and endosperm‐like motifs are widely distributed among plant promoters. Their putative role in nitrogen regulation is discussed.

Journal

The Plant JournalWiley

Published: Aug 1, 1993

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