THE NATURE OF THE AMINO ACID POOL USED FOR PROTEIN SYNTHESIS IN RAT BRAIN SLICES

THE NATURE OF THE AMINO ACID POOL USED FOR PROTEIN SYNTHESIS IN RAT BRAIN SLICES Abstract— The incorporation into brain slice protein of externally provided (1‐14C)valine was measured at varying levels of valine in the medium, under conditions of constant protein synthesis and equilibration of intracellular valine specific activity. The results indicate that the valine pool used for protein synthesis is not identical to the pool of total free valine. Neither does the incorporation solely occur from an extracellular pool which is in equilibrium with the incubation medium. The data are compatible with a two‐site activation model in which aminoacylation of tRNA occurs at both an internal site utilizing amino acid from the intracellular pool and an external (possibly membranous) site converting extracellular valine directly to valyl‐tRNA. A good fit to the experimental observations is also provided by a compartmented intracellular valine pool model. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

THE NATURE OF THE AMINO ACID POOL USED FOR PROTEIN SYNTHESIS IN RAT BRAIN SLICES

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Publisher
Wiley
Copyright
Copyright © 1979 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1979.tb00365.x
Publisher site
See Article on Publisher Site

Abstract

Abstract— The incorporation into brain slice protein of externally provided (1‐14C)valine was measured at varying levels of valine in the medium, under conditions of constant protein synthesis and equilibration of intracellular valine specific activity. The results indicate that the valine pool used for protein synthesis is not identical to the pool of total free valine. Neither does the incorporation solely occur from an extracellular pool which is in equilibrium with the incubation medium. The data are compatible with a two‐site activation model in which aminoacylation of tRNA occurs at both an internal site utilizing amino acid from the intracellular pool and an external (possibly membranous) site converting extracellular valine directly to valyl‐tRNA. A good fit to the experimental observations is also provided by a compartmented intracellular valine pool model.

Journal

Journal of NeurochemistryWiley

Published: Feb 1, 1979

References

  • Compartmentation of the inulin space in mouse brain slices
    Cohen, Cohen; Blasberg, Blasberg; Levi, Levi; Lajtha, Lajtha
  • Measurements of rates of protein synthesis in rat brain slices
    Dunlop, Dunlop; Elden, Elden; Lajtha, Lajtha
  • Amino acid distribution and incorporation into proteins in isolated, electrically‐stimulated cerebral tissues
    Jones, Jones; Ilwain, Ilwain
  • The effect of extracellular potassium concentration on protein synthesis in guinea‐pig hippocampal slices
    Lipton, Lipton; Heimbach, Heimbach
  • Measurements of in vivo rates of protein synthesis in brain, spinal cord, heart and liver of young versus adult, intact versus hypophysectomized rats
    Reith, Reith; Schotman, Schotman; Gispen, Gispen

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