Access the full text.
Sign up today, get DeepDyve free for 14 days.
J. Richards (1994)
Hormonal control of gene expression in the ovary.Endocrine reviews, 15 6
R. Bhandari, Hiroki Komuro, M. Higa, Masaru Nakamura (2004)
Sex Inversion of Sexually Immature Honeycomb Grouper (Epinephelus merra) by Aromatase Inhibitor, 21
G. Mor, M. Eliza, Joon Song, B. Wiita, Shiuan Chen, F. Naftolin (2001)
17α-Methyl testosterone is a competitive inhibitor of aromatase activity in Jar choriocarcinoma cells and macrophage-like THP-1 cells in cultureThe Journal of Steroid Biochemistry and Molecular Biology, 79
T. Sunobe, Masaru Nakamura, Yasuhisa Kobayashi, Tohru Kobayashi, Y. Nagahama (2005)
Aromatase immunoreactivity and the role of enzymes in steroid pathways for inducing sex change in the hermaphrodite gobiid fish Trimma okinawae.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 141 1
Yong Zhang, Weimin Zhang, Lihong Zhang, T. Zhu, Jing Tian, Xin Li, Hao-ran Lin (2004)
Two distinct cytochrome P450 aromatases in the orange-spotted grouper (Epinephelus coioides): cDNA cloning and differential mRNA expressionThe Journal of Steroid Biochemistry and Molecular Biology, 92
M. Hornung, K. Jensen, J. Korte, M. Kahl, E. Durhan, J. Denny, T. Henry, G. Ankley (2004)
Mechanistic basis for estrogenic effects in fathead minnow (Pimephales promelas) following exposure to the androgen 17alpha-methyltestosterone: conversion of 17alpha-methyltestosterone to 17alpha-methylestradiol.Aquatic toxicology, 66 1
R. Bhandari, M. Higa, Shigeo Nakamura, Masaru Nakamura (2004)
Aromatase inhibitor induces complete sex change in the protogynous honeycomb grouper (Epinephelus merra)Molecular Reproduction and Development, 67
Yan-Horn Lee, Jin-Lien Du, W. Yueh, Bih-Yun Lin, Jing-Duan Huang, Cheng-Yen Lee, Mong-Fong Lee, E. Lau, Fang-Yi Lee, C. Morrey, Yoshitaka Nagahama, Ching-Fong Chang (2001)
Sex change in the protandrous black porgy, Acanthopagrus schlegeli: a review in gonadal development, estradiol, estrogen receptor, aromatase activity and gonadotropin.The Journal of experimental zoology, 290 7
R. Bhandari, M. Alam, K. Soyano, Masaru Nakamura (2006)
Induction of Female-to–male Sex Change in the Honeycomb Grouper (Epinephelus merra) by 11-ketotestosterone Treatments, 23
Robert Viger, D. Silversides, J. Tremblay (2005)
New insights into the regulation of mammalian sex determination and male sex differentiation.Vitamins and hormones, 70
T. Kitano, K. Takamune, Y. Nagahama, S. Abe (2000)
Aromatase inhibitor and 17α‐methyltestosterone cause sex‐reversal from genetical females to phenotypic males and suppression of P450 aromatase gene expression in Japanese flounder (Paralichthys olivaceus)Molecular Reproduction and Development, 56
M. Amano, S. Hyodo, A. Urano, N. Okumoto, S. Kitamura, K. Ikuta, Y. Suzuki, K. Aida (1994)
Activation of salmon gonadotropin-releasing hormone synthesis by 17 alpha-methyltestosterone administration in yearling masu salmon, Oncorhynchus masou.General and comparative endocrinology, 95 3
K. Burns, C. Yan, T. Kumar, M. Matzuk (2001)
Analysis of ovarian gene expression in follicle-stimulating hormone beta knockout mice.Endocrinology, 142 7
M. Hornung, K. Jensen, J. Korte, M. Kahl, E. Durhan, J. Denny, T. Henry, G. Ankley (2003)
Mechanistic basis for estrogenic effects in fathead minnow (Pimephales promelas) following exposure to the androgen 17α-methyltestosterone: conversion of 17α-methyltestosterone to 17α-methylestradiolAquatic Toxicology, 66
J. Godwin, J. Luckenbach, R. Borski (2003)
Ecology meets endocrinology: environmental sex determination in fishesEvolution & Development, 5
M. Schmitz, S. Aroua, B. Vidal, N. Belle, P. Elie, S. Dufour (2005)
Differential Regulation of Luteinizing Hormone and Follicle-Stimulating Hormone Expression during Ovarian Development and under Sexual Steroid Feedback in the European EelNeuroendocrinology, 81
H. Kagawa, K. Gen, K. Okuzawa, Hideki Tanaka (2003)
Effects of Luteinizing Hormone and Follicle-Stimulating Hormone and Insulin-Like Growth Factor-I on Aromatase Activity and P450 Aromatase Gene Expression in the Ovarian Follicles of Red Seabream, Pagrus major1, 68
H. Kamei, T. Kaneko, K. Aida (2006)
Steroidogenic activities of follicle-stimulating hormone in the ovary of Japanese eel, Anguilla japonica.General and comparative endocrinology, 146 2
C. Kramer, M. Caddell, L. Bubenheimer‐Livolsi (1993)
sGnRH‐A [(d‐Arg6, Pro9, NEt‐)LHRH] in combination with domperidone induces gonad reversal in a protogynous fish, the bluehead wrasse, Thalassoma bifasciatumJournal of Fish Biology, 42
Shinn‐Lih Yeh, C. Kuo, Y. Ting, Ching-Fong Chang (2003)
Androgens stimulate sex change in protogynous grouper, Epinephelus coioides: spawning performance in sex-changed males.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 135C 3
R. Devlin, Y. Nagahama (2002)
Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influencesAquaculture, 208
Y. Yoshiura, B. Senthilkumaran, Masatada Watanabe, Y. Oba, Tohru Kobayashi, Y. Nagahama (2003)
Synergistic Expression of Ad4BP/SF-1 and Cytochrome P-450 Aromatase (Ovarian Type) in the Ovary of Nile Tilapia, Oreochromis niloticus, During Vitellogenesis Suggests Transcriptional Interaction1, 68
Yasuhisa Kobayashi, T. Sunobe, Tohru Kobayashi, Masaru Nakamura, N. Suzuki, Y. Nagahama (2005)
Molecular cloning and expression of Ad4BP/SF-1 in the serial sex changing gobiid fish, Trimma okinawae.Biochemical and biophysical research communications, 332 4
N. Montserrat, Alicia González, E. Méndez, F. Piferrer, J. Planas (2004)
Effects of follicle stimulating hormone on estradiol-17 beta production and P-450 aromatase (CYP19) activity and mRNA expression in brown trout vitellogenic ovarian follicles in vitro.General and comparative endocrinology, 137 2
B. Borg (1994)
Androgens in teleost fishesComparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 109
Shinn‐Lih Yeh, C. Kuo, Y. Ting, Ching-Fong Chang (2003)
The effects of exogenous androgens on ovarian development and sex change in female orange-spotted protogynous grouper, Epinephelus coioides.Aquaculture, 218
F. Tang, S. Chan, B. Lofts (1974)
Effect of mammalian luteinizing hormone on the natural sex reversal of the rice-field eel, Monopterus albus (Zuiew).General and comparative endocrinology, 24 3
Yan-Horn Lee, Fang-Yi Lee, W. Yueh, P. Tacon, Jin-Lien Du, Cheng-Nan Chang, S. Jeng, Hideki Tanaka, Ching-Fong Chang (2000)
Profiles of gonadal development, sex steroids, aromatase activity, and gonadotropin II in the controlled sex change of protandrous black porgy, Acanthopagrus schlegeli Bleeker.General and comparative endocrinology, 119 1
Yan-Horn Lee, W. Yueh, Jin-Lien Du, Lian-Tien Sun, Ching-Fong Chang (2002)
Aromatase Inhibitors Block Natural Sex Change and Induce Male Function in the Protandrous Black Porgy, Acanthopagrus schlegeli Bleeker: Possible Mechanism of Natural Sex Change1, 66
Y. Guiguen, J. Baroiller, M. Ricordel, K. Iseki, O. McMeel, Samuel Martin, A. Fostier (1999)
Involvement of estrogens in the process of sex differentiation in two fish species: The rainbow trout (Oncorhynchus mykiss) and a tilapia (Oreochromis niloticus)Molecular Reproduction and Development, 54
K. Burns, Changning Yan, T. Kumar, M. Matzuk (2001)
Analysis of Ovarian Gene Expression in Follicle-Stimulating Hormone β Knockout Mice.Endocrinology, 142 7
P. Val, A. LEFRANCOIS-MARTINEZ, G. Veyssière, Antoine Martinez (2003)
SF-1 a key player in the development and differentiation of steroidogenic tissuesNuclear Receptor, 1
Weimin Zhang, Xin Li, Yong Zhang, Lihong Zhang, Jing Tian, G. Ma (2004)
cDNA cloning and mRNA expression of a FTZ-F1 homologue from the pituitary of the orange-spotted grouper, epinephelus coioides.Journal of experimental zoology. Part A, Comparative experimental biology, 301 8
R. Bhandari, M. Alam, M. Higa, K. Soyano, Masaru Nakamura (2005)
Evidence that estrogen regulates the sex change of honeycomb grouper (Epinephelus merra), a protogynous hermaphrodite fish.Journal of experimental zoology. Part A, Comparative experimental biology, 303 6
Masatada Watanabe, Minoru Tanaka, D. Kobayashi, Y. Yoshiura, Y. Oba, Y. Nagahama (1999)
Medaka (Oryzias latipes) FTZ-F1 potentially regulates the transcription of P-450 aromatase in ovarian follicles: cDNA cloning and functional characterizationMolecular and Cellular Endocrinology, 149
Minoru Tanaka, S. Fukada, Michiya Matsuyama, Yoshitaka Nagahama (1995)
Structure and promoter analysis of the cytochrome P-450 aromatase gene of the teleost fish, medaka (Oryzias latipes).Journal of biochemistry, 117 4
W. Yeung, H. Chen, S. Chan (1993)
Effects of LH and LHRH-analog on gonadal development and in vitro steroidogenesis in the protogynous Monopterus albus.General and comparative endocrinology, 89 3
Masaru Nakamura, Tohru Kobayashi, X. Chang, Y. Nagahama (1998)
Gonadal sex differentiation in teleost fishJournal of Experimental Zoology, 281
The orange‐spotted grouper Epinephelus coioides is a protogynous hermaphroditic fish, but the physiological basis of its sex change remains largely unknown. In the present study, the 2‐year‐old orange‐spotted grouper was induced to change sex precociously by oral administration of 17α‐methyltestosterone (MT, 50 mg/Kg diet, twice a day at daily ration of 5% bodyweight) for 60 days. The serum testosterone levels were significantly elevated after MT treatment for 20 and 40 days as compared to control, but the levels of serum estradiol (E2) remained unchanged. The expression of P450aromA in the gonad significantly decreased after MT treatment for 20, 40, and 60 days. Accordingly, the enzyme activity of gonadal aromatase was also lower. The expression of FSHβ subunit in the pituitary was significantly decreased after MT treatment for 20 days, but returned to the control levels after 40 and 60 days; however, the expression of LHβ subunit was not altered significantly by MT treatment. The expression of FTZ‐F1 in the gonad also decreased significantly in response to MT treatment for 40 and 60 days, but its expression in the pituitary was not altered significantly. Interestingly, when tested in vitro on ovarian fragments, MT had no direct effect on the expression of P450aromA and FTZ‐F1 as well as the activity of gonadal aromatase, suggesting that the inhibition of gonadal P450aromatase and FTZ‐F1 by MT may be mediated at upper levels of the brain‐pituitary‐gonadal axis. Taken together, these results indicated that FSH, P450aromA, FTZ‐F1, and serum testosterone are associated with the MT‐induced sex change of the orange‐spotted grouper, but the cause–effect relationship between these factors and sex change in this species remains to be characterized. Mol. Reprod. Dev. 74: 665–673, 2007. © 2006 Wiley‐Liss, Inc.
Molecular Reproduction & Development – Wiley
Published: Jun 1, 2007
Keywords: teleost; protogynous hermaphroditism; semi‐quantitative RT‐PCR; testosterone; estradiol
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.