D. PAETKAU and C. STROBECK Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9 gesting that the mutation underlying the null alleles has existed for a reasonably long time. The molecular basis and phylogenetic origin of the null alleles was investigated by sequencing alleles of locus GlOP in each of the eight speaes of bears - two alleles from each species except for four alleles, including two null alleles, from each of U.nmericanus and U.thibetanus. The primers were a chimera of an M13 sequence primer and a microsatellite primer - a device which allowed collection of sequence data from the first base after the primer-binding site, as the start of the sequence reaction was moved back from the 3 end of the primer (AC-strand, â Introducation Non-amplifying, or ânullâ alleles at microsatelliteloci have been found to be common in humans (Callen et nl. 1993) and deer (Pemberton et al. 1995), and consideration must be given to the existence of such alleles in any microsatellite data set. The presence of segregating null alleles in populations is presumably the result of sequence polymorphisms that affect the binding site of one of the oligonucleotideprimers used for amplification.In one
Molecular Ecology – Wiley
Published: Aug 1, 1995
Keywords: ; ; ; ; ;
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