May-June 1985 Number 3 Invited Review The membrane and the lesions of storage in preserved red cells L. C. WOLFE ANANECDOTE RELATED by Dr. Diamond depicts the extent of the storage lesions in banked blood. Dr. J.G. Gibson, when he was studying blood preservatives, requested a specimen for his own analysis from a bottle of 21day old blood preserved in acid-citratedextrose (ACD) about to be used for transfusion. In error, the tube was sent to the hospital laboratory for routine analysis. The report returned as follows: This patient, whose red cells show extreme anisopoikilocytosis, must be suffering from a bizarre anemia that defies classification. It is obviously hemolytic, the plasma hemoglobin level being 150 mg%; 30% of red cells show markedly increased fragility and hemolysis; he is obviously diabetic, blood sugar being 450 mp%, also probably in renal failure with elevated plasma potassium and inorganic phosphate, as well as high BUN, also severe acidosis, the pH being 6.65. How long has this patient been dead?' Introduction When blood is collected in a standard anticoagulant-preservative solution and stored under blood bank conditions (i.e., in a standard bag set-up, in the presence of plasticizers and at a temperature of 4OC),
Transfusion – Wiley
Published: May 6, 1985
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