The intra‐cellular location of ABA was investigated in relation to its sites of synthesis. Chloroplasts were isolated from stressed and non‐stressed spinach leaves and their ABA content determined. Virtually all of the ABA from non‐stressed leaves was contained in the chloroplasts compared with only a small fraction of ABA isolated from stressed leaves. Chloroplasts prepared from turgid leaves and subsequently lysed in vitro retained most of their ABA and phaseic acid (PA) complement but this was removed with organic solvents. While the possibility of extra‐chloroplastic synthesis cannot be discounted the data indicate that stress‐induced ABA synthesis occurs in the chloroplast and that the ABA readily migrates from there to other parts of the plant.
Physiologia Plantarum – Wiley
Published: May 1, 1977
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