The use of nanomaterials in biological applications requires a better understanding of the effect of nanoparticles on the activity of proteins and related molecules. We have examined the influence of environmental particulate matter (PM) on the interaction of myoglobin and microperoxidase‐11 as model systems with the small molecules O2 and NO, respectively. The influence of PM on the reaction of dioxygen with deoxymyoglobin to form oxymyoglobin, and subsequently metmyoglobin, was studied for its essential role in living organisms. Samples of urban PM (SRM 1648a) were used as standard reference material. Also the influence of Fe2O3 nanoparticles on the stability of the investigated protein was studied. The obtained results show that binding and release of dioxygen by deoxymyoglobin may be affected by the presence of PM especially over longer periods of time. Similar studies were performed on the nitrosylation of microperoxidase‐11 as a model for the catalytic center of enzymes, for which the influence of the standard reference material, its water‐soluble extract, as well as metal oxide nanoparticles (Fe2O3 and ZnO), were studied. The reported results show that the presence of PM had no influence on the kinetics of the nitrosylation of the model microperoxidase under the selected experimental conditions.
European Journal of Inorganic Chemistry – Wiley
Published: Jan 14, 2018
Keywords: ; ; ; ;
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