Received: 29 December 2016 Accepted: 19 March 2017 Published on: 24 May 2017
The expression pattern of Bcl11a, Mdm2 and Pten genes
in B-cell acute lymphoblastic leukemia
Institute of Hematology, Medical College, Jinan
University, Guangzhou, PR China
Key Laboratory for Regenerative Medicine
of Ministry of Education, Jinan University,
Guangzhou, PR China
Yangqiu Li, Institute of Hematology, Medical
College and Key Laboratory for Regenerative
Medicine of Ministry of Education, Jinan Univer-
sity, Guangzhou, 510632, PR China.
Dongmei He, Institute of Hematology, Medical
College, Jinan University, Guangzhou 510632,
Aim: Bcl11a is closely associated with B-cell lymphoma and B-cell chronic lymphocytic leukemia.
However, little is known about the expression character of Bcl11a gene in B-cell acute lymphoblas-
tic leukemia (B-ALL). In previous study, our results showed there was a clear dysregulation in the
global gene expression of the Bcl11a-suppressed B lymphoma cells. The aim of the study is to fur-
ther evaluate the role of Bcl11a and the expression level of its related genes Mdm2, Pten in B-ALL
Methods: The relative mRNA expression levels of Bcl11a and Mdm2, Pten in blood mononuclear
cells of B-ALL patients were determined by real-time quantitative reverse transcript-polymerase
chain reaction (qRT-PCR) with SYBR Green Dye. Peripheral blood mononuclear cells from healthy
volunteers served as control. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was used as
Results: There was higher Bcl11a mRNA expression in initial diagnosed B-ALL than the healthy
control, but there is no statistical signiﬁcance (P > 0.05). The expression level of Bcl11a and Mdm2
in initial diagnosed B-ALL patients was statistically higher compared to those with complete remis-
sion (CR; P < 0.05). The expression levels of Bcl11a and Mdm2, Pten in B-ALL patients with CR were
decreased signiﬁcantly when compared with the healthy control (P < 0.05). The correlation anal-
yses revealed that the Bcl11a expression level was positively correlated with the Mdm2 and Pten
relative expression level in B-ALL patients. There is also positive correlation between Mdm2 and
Conclusions: Bcl11a transcript levels were signiﬁcantly downregulated in B-ALL patients with CR.
The decreased expression characteristics of Bcl11a and Mdm2, Pten might imply the CR of B-ALL
acute lymphoblastic leukemia, Bcl11a, B-cell, Mdm2, Pten, real-time quantitative reverse
transcript-polymerase chain reaction (qRT-PCR)
Human B-cell acute lymphoblastic leukemia (B-ALL) is characterized
by clonal expansion of developmentally arrested malignant B-cell pre-
cursors, accounting for 83% of ALLs and 30–40% of all childhood can-
cers. Despite our current knowledge of the cytogenetics of this disease,
obstacles to relapse-free survival remain for up to 15% of children and
the majority of adult with this disease.
B-cell chronic lymphocytic
leukemia (CLL)/lymphoma 11 a (Bcl11a) is a krüppel transcription fac-
tor that was initially discovered as a retroviral insertion site (Evi9)in
myeloid leukemia in the BXH-2 mouse, and closely related to B-cell
proliferation and differentiation.
Subsequently, Bcl11a was found to
be associated with human malignant B cells, overexpression of which is
primarily found in B-cell lymphoma and B-cell CLL.
The Bcl11a transcription factor is also aberrantly-expressed in
some persons with acute myeloid leukemia,
and increased in sub-
jects with chronic myeloid leukemia in acute phase compared with
those in normal and in subjects in chronic phase including some sub-
jects studied in both phases.
Our previous study also showed the
expression levels of Bcl11a in B CLL and B-cell lymphoma was signif-
icantly higher than those in the healthy control.
However, the role
of Bcl11a in B-ALL patients has not been reported. Meanwhile, our
previous results have shown that a broad range of genes including
murine double minute 2 (Mdm2) and phosphorate and tension homolog
2017 John Wiley & Sons Australia, Ltd Asia-Pac J Clin Oncol. 2018;14:e124–e128.wileyonlinelibrary.com/journal/ajco