Access the full text.
Sign up today, get DeepDyve free for 14 days.
Loading next page...
/lp/wiley/the-dota-protein-from-legionella-pneumophila-is-secreted-by-a-novel-dxNbE7PtfX
References (47)
-
J. Galán, A. Collmer (1999)
Type III secretion machines: bacterial devices for protein delivery into host cells.Science, 284 5418
-
C. Hueck (1998)
Type III Protein Secretion Systems in Bacterial Pathogens of Animals and PlantsMicrobiology and Molecular Biology Reviews, 62
-
K. Linton, C. Higgins (1998)
The Escherichia coli ATP‐binding cassette (ABC) proteinsMolecular Microbiology, 28
-
M. Homma, K. Kutsukake, T. Iino, S. Yamaguchi (1984)
Hook-associated proteins essential for flagellar filament formation in Salmonella typhimuriumJournal of Bacteriology, 157
-
T. Kubori, Yukiyasu Matsushima, D. Nakamura, Jaimol Uralil, M. Lara-Tejero, A. Sukhan, J. Galán, S. Aizawa (1998)
Supramolecular structure of the Salmonella typhimurium type III protein secretion system.Science, 280 5363
-
M. Sandkvist (2001)
Biology of type II secretionMolecular Microbiology, 40
-
P. Fekkes, A. Driessen (1999)
Protein Targeting to the Bacterial Cytoplasmic MembraneMicrobiology and Molecular Biology Reviews, 63
-
P. Christie (1997)
Agrobacterium tumefaciens T-complex transport apparatus: a paradigm for a new family of multifunctional transporters in eubacteriaJournal of Bacteriology, 179
-
T. Kubori, A. Sukhan, S. Aizawa, J. Galán (2000)
Molecular characterization and assembly of the needle complex of the Salmonella typhimurium type III protein secretion system.Proceedings of the National Academy of Sciences of the United States of America, 97 18
-
G. Segal, H. Shuman (1997)
Characterization of a new region required for macrophage killing by Legionella pneumophilaInfection and Immunity, 65
-
M. Horwitz (1983)
The Legionnaires' disease bacterium (Legionella pneumophila) inhibits phagosome-lysosome fusion in human monocytesThe Journal of Experimental Medicine, 158
-
M. Purcell, H. Shuman (1998)
The Legionella pneumophila icmGCDJBFGenes Are Required for Killing of Human MacrophagesInfection and Immunity, 66
-
Amanda Horstman, M. Kuehn (2000)
Enterotoxigenic Escherichia coli Secretes Active Heat-labile Enterotoxin via Outer Membrane Vesicles*The Journal of Biological Chemistry, 275
-
K. Berger, J. Merriam, R. Isberg (1994)
Altered intracellular targeting properties associated with mutations in the Legionella pneumophila dotA geneMolecular Microbiology, 14
-
K. Berger, R. Isberg (1993)
Two distinct defects in intracellular growth complemented by a single genetic locus in Legionella pneumophilaMolecular Microbiology, 7
-
K. Williams, M. Glibowicka, Zuomei Li, Hong Li, Amir Khan, Yv Chen, Jing Wang, D. Marvin, C. Deber (1995)
Packing of coat protein amphipathic and transmembrane helices in filamentous bacteriophage M13: role of small residues in protein oligomerization.Journal of molecular biology, 252 1
-
P. Christie, J. Vogel (2000)
Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells.Trends in microbiology, 8 8
-
M. Paetzel, R. Dalbey, N. Strynadka (2000)
The structure and mechanism of bacterial type I signal peptidases. A novel antibiotic target.Pharmacology & therapeutics, 87 1
-
K. Ferguson (1964)
STARCH-GEL ELECTROPHORESIS--APPLICATION TO THE CLASSIFICATION OF PITUITARY PROTEINS AND POLYPEPTIDES.Metabolism: clinical and experimental, 13
-
J. Coers, Jonathan Kagan, Miguelina Matthews, H. Nagai, Deborah Zuckman, C. Roy (2000)
Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growthMolecular Microbiology, 38
-
T. Komano, Tetsu Yoshida, Koji Narahara, N. Furuya (2000)
The transfer region of IncI1 plasmid R64: similarities between R64 tra and Legionella icm/dot genesMolecular Microbiology, 35
-
P. Christie (2001)
Type IV secretion: intercellular transfer of macromolecules by systems ancestrally related to conjugation machinesMolecular Microbiology, 40
-
B. Wilkins, A. Thomas (2000)
DNA‐independent transport of plasmid primase protein between bacteria by the I1 conjugation systemMolecular Microbiology, 38
-
J. Vogel, H. Andrews, S. Wong, R. Isberg (1998)
Conjugative transfer by the virulence system of Legionella pneumophila.Science, 279 5352
-
Frederick Quinn, Mark Keen, S. Lucy, Tompkins (1989)
Genetic, immunological, and cytotoxic comparisons of Legionella proteolytic activitiesInfection and Immunity, 57
-
H. Andrews, J. Vogel, R. Isberg (1998)
Identification of Linked Legionella pneumophila Genes Essential for Intracellular Growth and Evasion of the Endocytic PathwayInfection and Immunity, 66
-
A. Kuhn, W. Wickner (1985)
Isolation of mutants in M13 coat protein that affect its synthesis, processing, and assembly into phage.The Journal of biological chemistry, 260 29
-
L. Szeto, H. Shuman (1990)
The Legionella pneumophila major secretory protein, a protease, is not required for intracellular growth or cell killingInfection and Immunity, 58
-
Deborah Zuckman, Jiunn-Bin Hung, C. Roy (1999)
Pore‐forming activity is not sufficient for Legionella pneumophila phagosome trafficking and intracellular growthMolecular Microbiology, 32
-
L. Hales, H. Shuman (1999)
Legionella pneumophila Contains a Type II General Secretion Pathway Required for Growth in Amoebae as Well as for Secretion of the Msp ProteaseInfection and Immunity, 67
-
C. Roy, K. Berger, R. Isberg (1998)
Legionella pneumophila DotA protein is required for early phagosome trafficking decisions that occur within minutes of bacterial uptakeMolecular Microbiology, 28
-
J. Vogel, C. Roy, R. Isberg (1996)
Use of Salt to Isolate Legionella pneumophila Mutants Unable to Replicate in MacrophagesAnnals of the New York Academy of Sciences, 797
-
A. Pugsley (1993)
The complete general secretory pathway in gram-negative bacteria.Microbiological reviews, 57 1
-
G. Segal, H. Shuman (1999)
Possible origin of the Legionella pneumophila virulence genes and their relation to Coxiella burnetiiMolecular Microbiology, 33
-
M. Swanson, Andralph Isberg (1995)
Association of Legionella pneumophila with the macrophage endoplasmic reticulumInfection and Immunity, 63
-
B. Brand, A. Sadosky, H. Shuman (1994)
The Legionella pneumophila icm locus: a set of genes required for intracellular multiplication in human macrophagesMolecular Microbiology, 14
-
J. Feeley, R. Gibson, G. Gorman, Nancy Langford, J., Kamile Rasheed, D. Mackel, W. Baine (1979)
Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophilaJournal of Clinical Microbiology, 10
-
Daniel Clemens, Bai‐Yu Lee, M. Horwitz (2000)
Deviant Expression of Rab5 on Phagosomes Containing the Intracellular Pathogens Mycobacterium tuberculosis andLegionella pneumophila Is Associated with Altered Phagosomal FateInfection and Immunity, 68
-
G. Segal, M. Purcell, H. Shuman (1998)
Host cell killing and bacterial conjugation require overlapping sets of genes within a 22-kb region of the Legionella pneumophila genome.Proceedings of the National Academy of Sciences of the United States of America, 95 4
-
G. Plano, James Day, Franco Ferracci (2001)
Type III export: new uses for an old pathwayMolecular Microbiology, 40
-
J. Kirby, J. Vogel, H. Andrews, R. Isberg (1998)
Evidence for pore‐forming ability by Legionella pneumophilaMolecular Microbiology, 27
-
L. Wiater, K. Dunn, F. Maxfield, H. Shuman (1998)
Early Events in Phagosome Establishment Are Required for Intracellular Survival of Legionella pneumophilaInfection and Immunity, 66
-
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
-
Marjorie Russel, N. Linderoth, Andrej Sali (1997)
Filamentous phage assembly: variation on a protein export theme.Gene, 192 1
-
Miguelina Matthews, C. Roy (2000)
Identification and Subcellular Localization of the Legionella pneumophila IcmX Protein: a Factor Essential for Establishment of a Replicative Organelle in Eukaryotic Host CellsInfection and Immunity, 68
-
C. Roy, R. Isberg (1997)
Topology of Legionella pneumophila DotA: an inner membrane protein required for replication in macrophagesInfection and Immunity, 65
-
Andreas Kuhn (1995)
Major coat proteins of bacteriophage Pf3 and M13 as model systems for Sec-independent protein transport.FEMS microbiology reviews, 17 1-2
- Publisher
- Wiley
- Copyright
- Copyright © 2013 Wiley Periodicals, Inc
- ISSN
- 0261-4189
- eISSN
- 1460-2075
- DOI
- 10.1093/emboj/20.21.5962
- pmid
- 11689436
- Publisher site
- See Article on Publisher Site
Abstract
Legionella pneumophila requires the dot/icm genes to create an organelle inside eukaryotic host cells that will support bacterial replication. The dot/icm genes are predicted to encode a type IV‐related secretion apparatus. However, no proteins have been identified that require the dot/icm genes for secretion. In this study we show that the DotA protein, which was previously found to be a polytopic membrane protein, is secreted by the Dot/Icm transporter into culture supernatants. Secreted DotA protein was purified and N‐terminal sequencing of the purified protein revealed that a 19 amino acid leader peptide is removed from DotA prior to secretion. Extracellular DotA protein did not fractionate in membrane vesicles. Structures containing secreted DotA protein were visualized by electron microscopy and were shaped like hollow rings. These data indicate that the large poly topic membrane protein DotA is secreted from L.pneumophila by a unique process. This represents the first target secreted by the dot/icm‐encoded apparatus and demonstrates that this transporter is competent for protein secretion.
Journal
The EMBO Journal – Wiley
Published: Jan 1, 2001
Keywords: ; ; ; ;