The DOF protein, SAD, interacts with GAMYB in plant nuclei and activates transcription of endosperm‐specific genes during barley seed development

The DOF protein, SAD, interacts with GAMYB in plant nuclei and activates transcription of... Summary The DOF protein, SAD, previously shown to be a transcriptional activator in barley aleurone cells upon seed germination, also has an important role in gene regulation during endosperm development. mRNA was detected in early (10 days after flowering) developing barley seeds where it accumulated in the starchy endosperm, aleurone cells, nucellar projection, vascular tissues and the immature embryo, as shown by RT‐PCR and in situ hybridization analyses. The SAD protein, expressed in bacteria, binds to oligonucleotides containing the prolamine box, 5′‐A/TAAAG‐3′sequence, derived from the promoter regions of the endosperm‐specific genes Hor2 and Itr1, encoding a B‐hordein and trypsin‐inhibitor BTI‐CMe, respectively. SAD competed for the same binding sites with another endosperm‐expressed DOF protein, BPBF. Transient expression experiments in co‐bombarded developing endosperms demonstrated that SAD trans‐activated transcription from Hor2 and Itr1 promoters through binding to the intact DOF motifs. When the two DOF factors are co‐bombarded together an additive effect was observed upon the expression of the Itr1 gene. In‐frame fusion of the Sad ORF to the reporter green fluorescent protein gene directs the fluorescence expression to the nucleus in transiently transformed onion epidermal layers. The visualization of fluorescence in the nucleus of onion cells, using the bimolecular fluorescent complex (BiFC) approach, has shown the in vivo interaction between SAD and the R2R3MYB protein GAMYB. The interaction in plant cells has also been documented for the DOF protein BPBF and GAMYB, but nuclear interaction could not be detected between BPBF and SAD by this procedure. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

The DOF protein, SAD, interacts with GAMYB in plant nuclei and activates transcription of endosperm‐specific genes during barley seed development

Loading next page...
 
/lp/wiley/the-dof-protein-sad-interacts-with-gamyb-in-plant-nuclei-and-activates-ggg4wC2wx2
Publisher
Wiley
Copyright
Copyright © 2005 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
D.O.I.
10.1111/j.1365-313X.2005.02402.x
Publisher site
See Article on Publisher Site

Abstract

Summary The DOF protein, SAD, previously shown to be a transcriptional activator in barley aleurone cells upon seed germination, also has an important role in gene regulation during endosperm development. mRNA was detected in early (10 days after flowering) developing barley seeds where it accumulated in the starchy endosperm, aleurone cells, nucellar projection, vascular tissues and the immature embryo, as shown by RT‐PCR and in situ hybridization analyses. The SAD protein, expressed in bacteria, binds to oligonucleotides containing the prolamine box, 5′‐A/TAAAG‐3′sequence, derived from the promoter regions of the endosperm‐specific genes Hor2 and Itr1, encoding a B‐hordein and trypsin‐inhibitor BTI‐CMe, respectively. SAD competed for the same binding sites with another endosperm‐expressed DOF protein, BPBF. Transient expression experiments in co‐bombarded developing endosperms demonstrated that SAD trans‐activated transcription from Hor2 and Itr1 promoters through binding to the intact DOF motifs. When the two DOF factors are co‐bombarded together an additive effect was observed upon the expression of the Itr1 gene. In‐frame fusion of the Sad ORF to the reporter green fluorescent protein gene directs the fluorescence expression to the nucleus in transiently transformed onion epidermal layers. The visualization of fluorescence in the nucleus of onion cells, using the bimolecular fluorescent complex (BiFC) approach, has shown the in vivo interaction between SAD and the R2R3MYB protein GAMYB. The interaction in plant cells has also been documented for the DOF protein BPBF and GAMYB, but nuclear interaction could not be detected between BPBF and SAD by this procedure.

Journal

The Plant JournalWiley

Published: Jun 1, 2005

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off