—The uptake of 36Cl− into incubated synaptosomes was found to reach a steady state within 30 min enabling estimates of the amount of intra‐synaptosomal chloride to be made. There was an excess in the total charge of sodium and potassium over that of chloride within the synaptosomes. This deficit of permeant anions is balanced within the synaptosome by negatively charged protein and other anions such as aspartate and phosphate. Their combined amount could be estimated from the magnitude of the anion deficit. The net valency of the substances causing the anion deficit was determined by relating the ion content to the intra‐synaptosomal volume when metabolic activity was blocked. It was found to be similar to that of cerebral cortex tissue in vivo. The intra‐synaptosomal volume was found to change so that the osmolarity of the synaptosomal contents equalled the osmolarity of the incubation medium. A method of determining the intra‐synaptosomal volume by measuring the ion content is described. The results from this method are compared with those obtained using conventional space markers.
Journal of Neurochemistry – Wiley
Published: Oct 1, 1975
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera