THE CHLORIDE CONTENT, ANION DEFICIT AND VOLUME OF SYNAPTOSOMES

THE CHLORIDE CONTENT, ANION DEFICIT AND VOLUME OF SYNAPTOSOMES —The uptake of 36Cl− into incubated synaptosomes was found to reach a steady state within 30 min enabling estimates of the amount of intra‐synaptosomal chloride to be made. There was an excess in the total charge of sodium and potassium over that of chloride within the synaptosomes. This deficit of permeant anions is balanced within the synaptosome by negatively charged protein and other anions such as aspartate and phosphate. Their combined amount could be estimated from the magnitude of the anion deficit. The net valency of the substances causing the anion deficit was determined by relating the ion content to the intra‐synaptosomal volume when metabolic activity was blocked. It was found to be similar to that of cerebral cortex tissue in vivo. The intra‐synaptosomal volume was found to change so that the osmolarity of the synaptosomal contents equalled the osmolarity of the incubation medium. A method of determining the intra‐synaptosomal volume by measuring the ion content is described. The results from this method are compared with those obtained using conventional space markers. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

THE CHLORIDE CONTENT, ANION DEFICIT AND VOLUME OF SYNAPTOSOMES

Journal of Neurochemistry, Volume 25 (4) – Oct 1, 1975

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Publisher
Wiley
Copyright
Copyright © 1975 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1975.tb04351.x
Publisher site
See Article on Publisher Site

Abstract

—The uptake of 36Cl− into incubated synaptosomes was found to reach a steady state within 30 min enabling estimates of the amount of intra‐synaptosomal chloride to be made. There was an excess in the total charge of sodium and potassium over that of chloride within the synaptosomes. This deficit of permeant anions is balanced within the synaptosome by negatively charged protein and other anions such as aspartate and phosphate. Their combined amount could be estimated from the magnitude of the anion deficit. The net valency of the substances causing the anion deficit was determined by relating the ion content to the intra‐synaptosomal volume when metabolic activity was blocked. It was found to be similar to that of cerebral cortex tissue in vivo. The intra‐synaptosomal volume was found to change so that the osmolarity of the synaptosomal contents equalled the osmolarity of the incubation medium. A method of determining the intra‐synaptosomal volume by measuring the ion content is described. The results from this method are compared with those obtained using conventional space markers.

Journal

Journal of NeurochemistryWiley

Published: Oct 1, 1975

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