The Arabidopsis bZIP transcription factor HY5 regulates expression of the PFG1 / MYB12 gene in response to light and ultraviolet‐B radiation

The Arabidopsis bZIP transcription factor HY5 regulates expression of the PFG1 / MYB12 gene in... ABSTRACT Plants fend off potentially damaging ultraviolet (UV)‐B radiation by synthesizing and accumulating UV‐B‐absorbing flavonols that function as sunscreens. Regulation of this biosynthetic pathway is largely transcriptional and controlled by a network of transcription factors, among which the PRODUCTION OF FLAVONOL GLYCOSIDES (PFG) family of R2R3‐MYB transcription factors was recently identified with a pivotal function. Here, we describe the response of Arabidopsis seedlings to narrow‐band UV‐B radiation at the level of phenylpropanoid pathway genes using whole‐genome transcriptional profiling and identify the corresponding flavonol glycosides accumulating under UV‐B. We further show that the bZIP transcriptional regulator ELONGATED HYPOCOTYL5 (HY5) is required for the transcriptional activation of the PFG1/MYB12 and PFG3/MYB111 genes under UV‐B and visible light. A synthetic protein composed of HY5 with the VP16 activation domain is sufficient to activate PFG1/MYB12 expression in planta. However, even though myb11 myb12 myb111 triple mutants have strongly reduced CHS levels in darkness as well as in constant light, neither light‐ nor UV‐B‐inducibility seems impaired. Notwithstanding this, absence of the three PFG family transcription factors results in reduced UV‐B tolerance, whereas PFG1/MYB12 overexpression leads to an increased tolerance. Thus, our data suggest that HY5‐dependent regulation of PFG gene expression contributes to the establishment of UV‐B tolerance. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell & Environment Wiley

The Arabidopsis bZIP transcription factor HY5 regulates expression of the PFG1 / MYB12 gene in response to light and ultraviolet‐B radiation

Loading next page...
 
/lp/wiley/the-arabidopsis-bzip-transcription-factor-hy5-regulates-expression-of-TUhkZFtNqe
Publisher
Wiley
Copyright
© 2009 Blackwell Publishing Ltd
ISSN
0140-7791
eISSN
1365-3040
DOI
10.1111/j.1365-3040.2009.02061.x
Publisher site
See Article on Publisher Site

Abstract

ABSTRACT Plants fend off potentially damaging ultraviolet (UV)‐B radiation by synthesizing and accumulating UV‐B‐absorbing flavonols that function as sunscreens. Regulation of this biosynthetic pathway is largely transcriptional and controlled by a network of transcription factors, among which the PRODUCTION OF FLAVONOL GLYCOSIDES (PFG) family of R2R3‐MYB transcription factors was recently identified with a pivotal function. Here, we describe the response of Arabidopsis seedlings to narrow‐band UV‐B radiation at the level of phenylpropanoid pathway genes using whole‐genome transcriptional profiling and identify the corresponding flavonol glycosides accumulating under UV‐B. We further show that the bZIP transcriptional regulator ELONGATED HYPOCOTYL5 (HY5) is required for the transcriptional activation of the PFG1/MYB12 and PFG3/MYB111 genes under UV‐B and visible light. A synthetic protein composed of HY5 with the VP16 activation domain is sufficient to activate PFG1/MYB12 expression in planta. However, even though myb11 myb12 myb111 triple mutants have strongly reduced CHS levels in darkness as well as in constant light, neither light‐ nor UV‐B‐inducibility seems impaired. Notwithstanding this, absence of the three PFG family transcription factors results in reduced UV‐B tolerance, whereas PFG1/MYB12 overexpression leads to an increased tolerance. Thus, our data suggest that HY5‐dependent regulation of PFG gene expression contributes to the establishment of UV‐B tolerance.

Journal

Plant Cell & EnvironmentWiley

Published: Jan 1, 2010

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off