Systematic identification of genes expressed during early oogenesis in medaka

Systematic identification of genes expressed during early oogenesis in medaka Subtractive hybridization was used to identify differences in gene expression between medaka (Oryzias latipes) males and females during sex differentiation. Fifty female‐specific cDNA fragments were cloned. They can be classified into three groups by virtue of whether their earliest expression is at 1, 5, or 30 days after hatching. All 15 near full‐length cDNAs belonging to the first two groups were cloned. Many of these female‐specific genes are coordinately expressed in oocytes at the earliest stages of oogenesis. Some of the genes that were identified by their sequences include egg envelope proteins, oocyte‐specific RNA binding proteins, and a transcription factor containing a basic helix‐loop‐helix motif. Mol. Reprod. Dev. 55:31–36, 2000. © 2000 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Reproduction & Development Wiley

Systematic identification of genes expressed during early oogenesis in medaka

Molecular Reproduction & Development, Volume 55 (1) – Jan 1, 2000

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Publisher
Wiley
Copyright
Copyright © 2000 Wiley‐Liss, Inc.
ISSN
1040-452X
eISSN
1098-2795
DOI
10.1002/(SICI)1098-2795(200001)55:1<31::AID-MRD5>3.3.CO;2-Z
Publisher site
See Article on Publisher Site

Abstract

Subtractive hybridization was used to identify differences in gene expression between medaka (Oryzias latipes) males and females during sex differentiation. Fifty female‐specific cDNA fragments were cloned. They can be classified into three groups by virtue of whether their earliest expression is at 1, 5, or 30 days after hatching. All 15 near full‐length cDNAs belonging to the first two groups were cloned. Many of these female‐specific genes are coordinately expressed in oocytes at the earliest stages of oogenesis. Some of the genes that were identified by their sequences include egg envelope proteins, oocyte‐specific RNA binding proteins, and a transcription factor containing a basic helix‐loop‐helix motif. Mol. Reprod. Dev. 55:31–36, 2000. © 2000 Wiley‐Liss, Inc.

Journal

Molecular Reproduction & DevelopmentWiley

Published: Jan 1, 2000

Keywords: sex differentiation; subtractive hybridization; zona pellucida; transcription factor; oocyte

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