Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Systematic approaches to dissect biological processes in stem cells by image‐based screening

Systematic approaches to dissect biological processes in stem cells by image‐based screening High‐throughput RNAi or small molecule screens have proven to be powerful methodologies for the systematic dissection of cellular processes. In model organisms and cell lines, large‐scale screens have identified key components of many cellular pathways and helped to identify novel targets in disease‐relevant pathways. Image‐based high‐content screening has become an increasingly important tool in high‐throughput screening, enabling changes in phenotype characteristics, such as cell morphology and cell differentiation, to be monitored. In this review, we discuss the use of image‐based screening approaches to explore the behavior of adult, embryonic, and induced pluripotent stem cells. First, we review how current pluripotency and differentiation assays can be adapted to high‐throughput formats. We then describe general aspects of image‐based screening of cells and present an outlook on challenges for screening stem cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Journal Wiley

Systematic approaches to dissect biological processes in stem cells by image‐based screening

Loading next page...
 
/lp/wiley/systematic-approaches-to-dissect-biological-processes-in-stem-cells-by-GzXp0zPB1M

References (113)

Publisher
Wiley
Copyright
Copyright © 2012 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1860-6768
eISSN
1860-7314
DOI
10.1002/biot.201200117
pmid
22653826
Publisher site
See Article on Publisher Site

Abstract

High‐throughput RNAi or small molecule screens have proven to be powerful methodologies for the systematic dissection of cellular processes. In model organisms and cell lines, large‐scale screens have identified key components of many cellular pathways and helped to identify novel targets in disease‐relevant pathways. Image‐based high‐content screening has become an increasingly important tool in high‐throughput screening, enabling changes in phenotype characteristics, such as cell morphology and cell differentiation, to be monitored. In this review, we discuss the use of image‐based screening approaches to explore the behavior of adult, embryonic, and induced pluripotent stem cells. First, we review how current pluripotency and differentiation assays can be adapted to high‐throughput formats. We then describe general aspects of image‐based screening of cells and present an outlook on challenges for screening stem cells.

Journal

Biotechnology JournalWiley

Published: Jun 1, 2012

Keywords: ; ; ; ;

There are no references for this article.