Survival of purified embryonic chick retinal ganglion cells in the presence of neurotrophic factors

Survival of purified embryonic chick retinal ganglion cells in the presence of neurotrophic factors In a search for neurotrophic factors (NTFs) regulating retinal ganglion cell (RGC) death in the chick embryo we have used purified and cultured RGCs. Purification of RGCs from embryonic day 10 was achieved by employing the “panning” method (Silverstein and Chun: Soc Neurosci Abstr 13:1054, 1987). The obtained neuron population consisted of 97% RGCs as demonstrated by retrograde labeling with a fluorescence dye. RGCs were cultured at low density in a chemically defined medium and shortterm survival (24 hr) was determined. In the absence of NTFs, less than 3% of the RGCs survived. In the presence of various crude or purified NTFs (eye, brain, and tectum extracts; glial‐conditioned medium; ciliary nerotrophic factor (CNTF); nerve growth factor (NGF)) 31% to 52% of the RGCs were maintained. The effects of NGF and CNTF were not additive. Neither acidic nor basic fibroblast growth factor was able to maintain RGCs in culture. Our results, obtained with a culture system which allowed the analysis of direct trophic actions, suggest that NGF and CNTF may be NTFs for overlapping subpopulations of chick RGCs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Research Wiley

Survival of purified embryonic chick retinal ganglion cells in the presence of neurotrophic factors

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Publisher
Wiley
Copyright
Copyright © 1989 Alan R. Liss, Inc.
ISSN
0360-4012
eISSN
1097-4547
DOI
10.1002/jnr.490240225
Publisher site
See Article on Publisher Site

Abstract

In a search for neurotrophic factors (NTFs) regulating retinal ganglion cell (RGC) death in the chick embryo we have used purified and cultured RGCs. Purification of RGCs from embryonic day 10 was achieved by employing the “panning” method (Silverstein and Chun: Soc Neurosci Abstr 13:1054, 1987). The obtained neuron population consisted of 97% RGCs as demonstrated by retrograde labeling with a fluorescence dye. RGCs were cultured at low density in a chemically defined medium and shortterm survival (24 hr) was determined. In the absence of NTFs, less than 3% of the RGCs survived. In the presence of various crude or purified NTFs (eye, brain, and tectum extracts; glial‐conditioned medium; ciliary nerotrophic factor (CNTF); nerve growth factor (NGF)) 31% to 52% of the RGCs were maintained. The effects of NGF and CNTF were not additive. Neither acidic nor basic fibroblast growth factor was able to maintain RGCs in culture. Our results, obtained with a culture system which allowed the analysis of direct trophic actions, suggest that NGF and CNTF may be NTFs for overlapping subpopulations of chick RGCs.

Journal

Journal of Neuroscience ResearchWiley

Published: Oct 1, 1989

References

  • Purification of the chick eye ciliary neuronotrophic factor
    Barbin, Barbin; Manthorpe, Manthorpe; Varon, Varon
  • Partial characterization of chicken Thy‐1 glycoprotein by monoclonal antibodies
    French, French; Jeffrey, Jeffrey
  • Ciliary neuronotrophic factor stimulates choline acetyltransferase activity in cultured chicken retina neurons
    Hofmann, Hofmann
  • Survival and axonal elongation of adult rat retinal ganglion cells
    Thanos, Thanos; Baehr, Baehr; Barde, Barde; Vanselow, Vanselow
  • Neuronotrophic factors released by C6 glioma cells
    Westermann, Westermann; Hardung, Hardung; Meyer, Meyer; Ehrhard, Ehrhard; Otten, Otten; Unsicker, Unsicker

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