Supraspinal delta opioid receptor mRNA levels are not altered in (D‐Ala 2 )Deltorphin II tolerant mice

Supraspinal delta opioid receptor mRNA levels are not altered in (D‐Ala 2 )Deltorphin II... Delta opioid receptor (DOR) mRNA levels were studied in mice rendered tolerant to (D‐Ala2)deltorphin II by 4 days of repeated intracerebroventricular administration (10μg, (tid)). ED50 determinations on day 5 revealed a 10‐fold loss in (D‐Ala2)deltorphin II potency with the tail‐flick test. Utilization of a microdissection technique followed by quantitative solution hybridization of RNA extracts from mouse brain revealed mean levels of DOR mRNA ranging from 3.9 pg/μg RNA in the caudate‐putamen to 0.4 pg/μg RNA in the cerebellum. DOR mRNA levels were not different when RNA extracts from tolerant and nontolerant mice were compared. These data suggest that altered DOR mRNA levels are not one of the adaptive changes that occur with delta opioid ((D‐Ala2)deltorphin II) tolerance. © 1994 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Research Wiley

Supraspinal delta opioid receptor mRNA levels are not altered in (D‐Ala 2 )Deltorphin II tolerant mice

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Publisher
Wiley
Copyright
Copyright © 1994 Wiley‐Liss, Inc.
ISSN
0360-4012
eISSN
1097-4547
D.O.I.
10.1002/jnr.490390608
Publisher site
See Article on Publisher Site

Abstract

Delta opioid receptor (DOR) mRNA levels were studied in mice rendered tolerant to (D‐Ala2)deltorphin II by 4 days of repeated intracerebroventricular administration (10μg, (tid)). ED50 determinations on day 5 revealed a 10‐fold loss in (D‐Ala2)deltorphin II potency with the tail‐flick test. Utilization of a microdissection technique followed by quantitative solution hybridization of RNA extracts from mouse brain revealed mean levels of DOR mRNA ranging from 3.9 pg/μg RNA in the caudate‐putamen to 0.4 pg/μg RNA in the cerebellum. DOR mRNA levels were not different when RNA extracts from tolerant and nontolerant mice were compared. These data suggest that altered DOR mRNA levels are not one of the adaptive changes that occur with delta opioid ((D‐Ala2)deltorphin II) tolerance. © 1994 Wiley‐Liss, Inc.

Journal

Journal of Neuroscience ResearchWiley

Published: Dec 15, 1994

References

  • Role of receptor regulation in opioid tolerance mechanisms
    Loh, Loh; Tao, Tao; Smith, Smith

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