The recent advances in the resolution obtained by single‐particle reconstructions from cryo‐electron microscopy (cryo‐EM) have led to an increase in studies that combine X‐ray crystallographic results with those of electron microscopy (EM). Here, such a combination is described in the determination of the structure of an enveloped animal virus, Semliki Forest virus, at 9 Å resolution. The issues of model bias in determination of the structure, the definition of resolution in a single‐particle reconstruction, the effect of the correction of the contrast‐transfer function on the structure determined and the use of a high‐resolution structure of a subunit in the interpretation of the structure of the complex are addressed.
Acta Crystallographica Section D – Wiley
Published: Oct 1, 2000
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