Supplanting crystallography or supplementing microscopy? A combined approach to the study of an enveloped virus

Supplanting crystallography or supplementing microscopy? A combined approach to the study of an... The recent advances in the resolution obtained by single‐particle reconstructions from cryo‐electron microscopy (cryo‐­EM) have led to an increase in studies that combine X‐ray crystallographic results with those of electron microscopy (EM). Here, such a combination is described in the determination of the structure of an enveloped animal virus, Semliki Forest virus, at 9 Å resolution. The issues of model bias in determination of the structure, the definition of resolution in a single‐particle reconstruction, the effect of the correction of the contrast‐transfer function on the structure determined and the use of a high‐resolution structure of a subunit in the interpretation of the structure of the complex are addressed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Crystallographica Section D Wiley

Supplanting crystallography or supplementing microscopy? A combined approach to the study of an enveloped virus

Loading next page...
 
/lp/wiley/supplanting-crystallography-or-supplementing-microscopy-a-combined-7xjVdDwWwP
Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1399-0047
eISSN
1399-0047
DOI
10.1107/S0907444900010817
Publisher site
See Article on Publisher Site

Abstract

The recent advances in the resolution obtained by single‐particle reconstructions from cryo‐electron microscopy (cryo‐­EM) have led to an increase in studies that combine X‐ray crystallographic results with those of electron microscopy (EM). Here, such a combination is described in the determination of the structure of an enveloped animal virus, Semliki Forest virus, at 9 Å resolution. The issues of model bias in determination of the structure, the definition of resolution in a single‐particle reconstruction, the effect of the correction of the contrast‐transfer function on the structure determined and the use of a high‐resolution structure of a subunit in the interpretation of the structure of the complex are addressed.

Journal

Acta Crystallographica Section DWiley

Published: Oct 1, 2000

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off