Studies on the tumor‐promoting activity of additives in biomaterials: Inhibition of metabolic cooperation by phenolic antioxidants involved in rubber materials

Studies on the tumor‐promoting activity of additives in biomaterials: Inhibition of metabolic... For the detection of tumor‐promoting activities of phenolic antioxidants, the inhibitory activities on the intercellular gap‐junctional communication were investigated using the V79 metabolic cooperation (MC) assay. Among eight antioxidants, 4,4′‐butylidene‐bis(3‐methyl‐6‐tert‐butyl‐phenol), 2,2′‐methylene‐bis(4‐methyl‐6‐tert‐butylphenol) (MBMBP), and styrenated phenol (SP) showed stronger inhibitory activities than lithocholic acid, which is known to be a tumor promotor. However, 4,4′‐thio‐bis(3‐methyl‐6‐tert‐butylphenol), Irganox 1010, and 1330 did not inhibit at any concentrations. When the single‐electron oxidation potentials were compared among antioxidants, the electrochemical ease estimated with the first oxidation potential was correlated with the cytotoxic potentials (r = 0.88), but not with the inhibitory activities in an MC assay. The tumor‐promoting activity of MBMBP was also investigated using an in vitro, two‐stage Balb/c 3T3 transformation assay. MBMBP did not show initiating activity, but significant promoting activity at concentrations of both 1 and 2.5 μg/ml were noted. These concentrations were close to the lowest effective inhibitory concentration (1.3 μg/ml) of MBMBP in an MC assay. In conclusion, there is a possibility that the phenolic antioxidants that show inhibitory activities in an MC assay contribute to the enhancement of tumor incidence induced by biomaterials. © 1995 John Wiley & Sons, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biomedical Materials Research Part A Wiley

Studies on the tumor‐promoting activity of additives in biomaterials: Inhibition of metabolic cooperation by phenolic antioxidants involved in rubber materials

Loading next page...
 
/lp/wiley/studies-on-the-tumor-promoting-activity-of-additives-in-biomaterials-39rxpI7DTc
Publisher
Wiley
Copyright
Copyright © 1995 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1549-3296
eISSN
1552-4965
DOI
10.1002/jbm.820290117
Publisher site
See Article on Publisher Site

Abstract

For the detection of tumor‐promoting activities of phenolic antioxidants, the inhibitory activities on the intercellular gap‐junctional communication were investigated using the V79 metabolic cooperation (MC) assay. Among eight antioxidants, 4,4′‐butylidene‐bis(3‐methyl‐6‐tert‐butyl‐phenol), 2,2′‐methylene‐bis(4‐methyl‐6‐tert‐butylphenol) (MBMBP), and styrenated phenol (SP) showed stronger inhibitory activities than lithocholic acid, which is known to be a tumor promotor. However, 4,4′‐thio‐bis(3‐methyl‐6‐tert‐butylphenol), Irganox 1010, and 1330 did not inhibit at any concentrations. When the single‐electron oxidation potentials were compared among antioxidants, the electrochemical ease estimated with the first oxidation potential was correlated with the cytotoxic potentials (r = 0.88), but not with the inhibitory activities in an MC assay. The tumor‐promoting activity of MBMBP was also investigated using an in vitro, two‐stage Balb/c 3T3 transformation assay. MBMBP did not show initiating activity, but significant promoting activity at concentrations of both 1 and 2.5 μg/ml were noted. These concentrations were close to the lowest effective inhibitory concentration (1.3 μg/ml) of MBMBP in an MC assay. In conclusion, there is a possibility that the phenolic antioxidants that show inhibitory activities in an MC assay contribute to the enhancement of tumor incidence induced by biomaterials. © 1995 John Wiley & Sons, Inc.

Journal

Journal of Biomedical Materials Research Part AWiley

Published: Jan 1, 1995

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off