Studies of Erythrocyte Membrane Loss Produced by Amphipathic Drugs and in Vitro Storage

Studies of Erythrocyte Membrane Loss Produced by Amphipathic Drugs and in Vitro Storage Four amphipathic drugs, primaquine, propranolol, chlorpromazine and tetracaine, were used to cause endocytosis in glucose‐depleted red cells, and the relative reduction of membrane surfaces was measured by the toluidine blue (TB) method. The TB measurements correlated well with the observed electron microscopic alterations. In vitro blood storage studies indicated loss of cell membrane during storage correlated with decreasing uptake of TB by the red cells. Regeneration of cellular ATP with adenosine did not always restore TB uptake by the cells. Loss of red blood cell membrane either by exocytosis or endocytosis may occur during normal in vivo or in vitro ageing, and may be increased in pathological states or by the action of drugs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png British Journal of Haematology Wiley

Studies of Erythrocyte Membrane Loss Produced by Amphipathic Drugs and in Vitro Storage

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Abstract

Four amphipathic drugs, primaquine, propranolol, chlorpromazine and tetracaine, were used to cause endocytosis in glucose‐depleted red cells, and the relative reduction of membrane surfaces was measured by the toluidine blue (TB) method. The TB measurements correlated well with the observed electron microscopic alterations. In vitro blood storage studies indicated loss of cell membrane during storage correlated with decreasing uptake of TB by the red cells. Regeneration of cellular ATP with adenosine did not always restore TB uptake by the cells. Loss of red blood cell membrane either by exocytosis or endocytosis may occur during normal in vivo or in vitro ageing, and may be increased in pathological states or by the action of drugs.

Journal

British Journal of HaematologyWiley

Published: Aug 1, 1978

References

  • Evaluation of toluidine blue for measuring membrane loss during in vivo ageing
    Greenwalt, Greenwalt; Lau, Lau
  • Alteration of the conformation of proteins in red blood cell membranes and in solution by fixatives used in electron microscopy
    Lenard, Lenard; Singer, Singer

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