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Student research award in the undergraduate, master candidate, or health science degree candidate category, 15th annual meeting of the society for biomaterials, Lake Buena Vista, Florida, April 28–may 2, 1989. Toxicity of copper‐based dental alloys in cell culture

Student research award in the undergraduate, master candidate, or health science degree candidate... The biocompatibility of three commercial copper‐based dental casting alloys—Duracast MS, Goldent, and Trindium—three experimental copper alloys, and a control gold alloy, Modulay, were investigated. Trindium, Duracast MS, experimental alloys 1 and 2 are aluminum bronzes; Goldent is a hybrid aluminum–brass alloy; and experimental alloy #3 is a high zinc brass alloy. ASTM F813–83 Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices, a 3‐day direct‐contact cell culture regimen and atomic absorption spectroscopy were utilized for evaluating the biocompatibility of these alloys in both Waymouth's and RPMI 1640 complete media. Cellular proliferation assays, using 3H‐thymidine uptake, were also conducted in Waymouth's media. In this investigation, only the experimental alloy #3 elicited alterations in morphology and viability of the fibroblast monolayer during the ASTM and 3‐day culture tests in either media. Cell cultures exposed to experimental alloy #3 experienced copper concentrations greater than 16.0 ppm in Waymouth's and 10 ppm copper in RPMI 1640 media. Differences in the size of the cytotoxic zone around experimental alloy #3 were also observed, with the larger zone occurring in Waymouth's media. In contrast to the direct cell contact studies, all alloys caused decreases in 3H‐thymidine uptake in Waymouth's media at much reduced metal ion concentrations as compared to the controls. Thus, adverse changes in DNA synthesis occurred at much lower copper and zinc concentrations than changes in morphology and viability. Consequently, the assessment of biocompatibility is dependent on the parameters evaluated, and several parameters must be analyzed before a material may be considered biocompatible. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biomedical Materials Research Part A Wiley

Student research award in the undergraduate, master candidate, or health science degree candidate category, 15th annual meeting of the society for biomaterials, Lake Buena Vista, Florida, April 28–may 2, 1989. Toxicity of copper‐based dental alloys in cell culture

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References (2)

Publisher
Wiley
Copyright
Copyright © 1989 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1549-3296
eISSN
1552-4965
DOI
10.1002/jbm.820231002
pmid
2808459
Publisher site
See Article on Publisher Site

Abstract

The biocompatibility of three commercial copper‐based dental casting alloys—Duracast MS, Goldent, and Trindium—three experimental copper alloys, and a control gold alloy, Modulay, were investigated. Trindium, Duracast MS, experimental alloys 1 and 2 are aluminum bronzes; Goldent is a hybrid aluminum–brass alloy; and experimental alloy #3 is a high zinc brass alloy. ASTM F813–83 Standard Practice for Direct Contact Cell Culture Evaluation of Materials for Medical Devices, a 3‐day direct‐contact cell culture regimen and atomic absorption spectroscopy were utilized for evaluating the biocompatibility of these alloys in both Waymouth's and RPMI 1640 complete media. Cellular proliferation assays, using 3H‐thymidine uptake, were also conducted in Waymouth's media. In this investigation, only the experimental alloy #3 elicited alterations in morphology and viability of the fibroblast monolayer during the ASTM and 3‐day culture tests in either media. Cell cultures exposed to experimental alloy #3 experienced copper concentrations greater than 16.0 ppm in Waymouth's and 10 ppm copper in RPMI 1640 media. Differences in the size of the cytotoxic zone around experimental alloy #3 were also observed, with the larger zone occurring in Waymouth's media. In contrast to the direct cell contact studies, all alloys caused decreases in 3H‐thymidine uptake in Waymouth's media at much reduced metal ion concentrations as compared to the controls. Thus, adverse changes in DNA synthesis occurred at much lower copper and zinc concentrations than changes in morphology and viability. Consequently, the assessment of biocompatibility is dependent on the parameters evaluated, and several parameters must be analyzed before a material may be considered biocompatible.

Journal

Journal of Biomedical Materials Research Part A – Wiley

Published: Oct 1, 1989

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